PDBsum entry 3wz3

Unknown function

PDB id: 3wz3

Contents

Protein chain

137 a.a.

Ligands

SO4

Waters ×230

PDB id:

3wz3

Name:

Unknown function

Title:

Structure of a periplasmic fragment of tram

Structure:

Tram protein. Chain: a. Fragment: periplasmic fragment, unp residues 91-230. Engineered: yes

Source:

Plasmid r64. Organism_taxid: 2492. Gene: tram. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.50Å R-factor: 0.182 R-free: 0.207

Authors:

T.Kuroda,T.Kubori,Y.Uchida,H.Nagai,K.Imada

Key ref:

T.Kuroda et al. (2015). Molecular and structural analysis of Legionella DotI gives insights into an inner membrane complex essential for type IV secretion. Sci Rep, 5, 10912. PubMed id: 26039110 DOI: 10.1038/srep10912

Date:

18-Sep-14 Release date: 10-Jun-15

Protein chain

Q9R2H2  (Q9R2H2_9ZZZZ) -  TraM protein from Plasmid R64

Seq: 230 a.a.

Struc: 137 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1038/srep10912

Sci Rep 5:10912 (2015)

PubMed id: 26039110

Molecular and structural analysis of Legionella DotI gives insights into an inner membrane complex essential for type IV secretion.

T.Kuroda, T.Kubori, X.Thanh Bui, A.Hyakutake, Y.Uchida, K.Imada, H.Nagai.

ABSTRACT

The human pathogen Legionella pneumophila delivers a large array of the effector proteins into host cells using the Dot/Icm type IVB secretion system. Among the proteins composing the Dot/Icm system, an inner membrane protein DotI is known to be crucial for the secretion function but its structure and role in type IV secretion had not been elucidated. We report here the crystal structures of the periplasmic domains of DotI and its ortholog in the conjugation system of plasmid R64, TraM. These structures reveal a striking similarity to VirB8, a component of type IVA secretion systems, suggesting that DotI/TraM is the type IVB counterpart of VirB8. We further show that DotI and its partial paralog DotJ form a stable heterocomplex. R64 TraM, encoded by the conjugative plasmid lacking DotJ ortholog, forms a homo-hexamer. The DotI-DotJ complex is distinct from the core complex, which spans both inner and outer membranes to form a substrate conduit, and seems not to stably associate with the core complex. These results give insight into VirB8-family inner membrane proteins essential for type IV secretion and aid towards understanding the molecular basis of secretion systems essential for bacterial pathogenesis.