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PDBsum entry 3wz3
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Unknown function
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PDB id
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3wz3
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References listed in PDB file
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Key reference
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Title
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Molecular and structural analysis of legionella doti gives insights into an inner membrane complex essential for type iv secretion.
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Authors
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T.Kuroda,
T.Kubori,
X.Thanh bui,
A.Hyakutake,
Y.Uchida,
K.Imada,
H.Nagai.
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Ref.
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Sci Rep, 2015,
5,
10912.
[DOI no: ]
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PubMed id
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Abstract
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The human pathogen Legionella pneumophila delivers a large array of the effector
proteins into host cells using the Dot/Icm type IVB secretion system. Among the
proteins composing the Dot/Icm system, an inner membrane protein DotI is known
to be crucial for the secretion function but its structure and role in type IV
secretion had not been elucidated. We report here the crystal structures of the
periplasmic domains of DotI and its ortholog in the conjugation system of
plasmid R64, TraM. These structures reveal a striking similarity to VirB8, a
component of type IVA secretion systems, suggesting that DotI/TraM is the type
IVB counterpart of VirB8. We further show that DotI and its partial paralog DotJ
form a stable heterocomplex. R64 TraM, encoded by the conjugative plasmid
lacking DotJ ortholog, forms a homo-hexamer. The DotI-DotJ complex is distinct
from the core complex, which spans both inner and outer membranes to form a
substrate conduit, and seems not to stably associate with the core complex.
These results give insight into VirB8-family inner membrane proteins essential
for type IV secretion and aid towards understanding the molecular basis of
secretion systems essential for bacterial pathogenesis.
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