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PDBsum entry 3v0c
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PDB id:
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Toxin
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Title:
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4.3 angstrom crystal structure of an inactive bont/a (e224q/r363a/y366f)
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Structure:
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Bont/a. Chain: a. Fragment: inactive full length bont/a1. Synonym: bont/a1, botulinum neurotoxin type a, neurotoxin a, neurotoxin bont. Engineered: yes. Mutation: yes
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Source:
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Clostridium botulinum. Organism_taxid: 1491. Gene: bont/a, bont/a, bonta. Expressed in: escherichia coli. Expression_system_taxid: 562
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Resolution:
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4.30Å
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R-factor:
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0.324
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R-free:
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0.349
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Authors:
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S.Gu,S.Rumpel,J.Zhou,J.Strotmeier,H.Bigalke,K.Perry,C.B.Shoemaker, A.Rummel,R.Jin
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Key ref:
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S.Gu
et al.
(2012).
Botulinum neurotoxin is shielded by NTNHA in an interlocked complex.
Science,
335,
977-981.
PubMed id:
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Date:
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07-Dec-11
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Release date:
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14-Mar-12
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PROCHECK
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Headers
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References
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P0DPI1
(BXA1_CLOBH) -
Botulinum neurotoxin type A from Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
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Seq:
Struc:
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1296 a.a.
1277 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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*
PDB and UniProt seqs differ
at 4 residue positions (black
crosses)
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Enzyme class:
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E.C.3.4.24.69
- bontoxilysin.
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Reaction:
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Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevins, SNAP25 or syntaxin. No detected action on small molecule substrates.
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Cofactor:
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Zn(2+)
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Science
335:977-981
(2012)
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PubMed id:
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Botulinum neurotoxin is shielded by NTNHA in an interlocked complex.
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S.Gu,
S.Rumpel,
J.Zhou,
J.Strotmeier,
H.Bigalke,
K.Perry,
C.B.Shoemaker,
A.Rummel,
R.Jin.
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ABSTRACT
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