PDBsum entry 3otc

Transferase

PDB id

3otc

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Contents

			Protein chain

					264 a.a. *

* Residue conservation analysis

PDB id:

3otc

Links

Name:

Transferase

Title:

Crystal structure of human trnahis guanylyltransferase (thg1)- native ii

Structure:

tRNA(his) guanylyltransferase. Chain: a, b. Fragment: unp residues 30-298. Synonym: tRNA-histidine guanylyltransferase, interphase cytoplasmic foci protein 45. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Strain: kato iii. Gene: icf45, thg1l. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

3.01Å

R-factor:

0.257

R-free:

0.278

Authors:

S.J.Hyde,B.E.Eckenroth,S.Doublie

Key ref:

S.J.Hyde et al. (2010). tRNA(His) guanylyltransferase (THG1), a unique 3'-5' nucleotidyl transferase, shares unexpected structural homology with canonical 5'-3' DNA polymerases. Proc Natl Acad Sci U S A, 107, 20305-20310. PubMed id: 21059936

Date:

11-Sep-10

Release date:

17-Nov-10

PROCHECK

	Headers

	References

Protein chains

Q9NWX6 (THG1_HUMAN) - Probable tRNA(His) guanylyltransferase from Homo sapiens

Seq: Struc:					298 a.a. 264 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.2.7.7.79 - tRNA(His) guanylyltransferase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

a 5'-end ribonucleotide-tRNA(His) + GTP + ATP + H2O = a 5'-end phospho- guanosine-ribonucleotide-tRNA(His) + AMP + 2 diphosphate + H⁺

5'-end ribonucleotide-tRNA(His)	+	GTP	+	ATP	+	H2O	=	5'-end phospho- guanosine-ribonucleotide-tRNA(His)	+	AMP	+	2 × diphosphate	+	H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

	Proc Natl Acad Sci U S A 107:20305-20310 (2010)
PubMed id: 21059936

tRNA(His) guanylyltransferase (THG1), a unique 3'-5' nucleotidyl transferase, shares unexpected structural homology with canonical 5'-3' DNA polymerases.
S.J.Hyde, B.E.Eckenroth, B.A.Smith, W.A.Eberley, N.H.Heintz, J.E.Jackman, S.Doublié.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21078997

J.J.Perona, and J.P.Oza (2010).
Crystal structure of a reverse polymerase.

Proc Natl Acad Sci U S A, 107, 20149-20150.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.