PDBsum entry 3n2k

Cell cycle

PDB id: 3n2k

Contents

Protein chains

428 a.a. *

420 a.a. *

123 a.a. *

Ligands

GTP ×2

GDP ×2

K2N ×2

Metals

_MG ×3

* Residue conservation analysis

PDB id:

3n2k

Name:

Cell cycle

Title:

Tubulin-nsc 613862: rb3 stathmin-like domain complex

Structure:

Tubulin alpha chain. Chain: a, c. Tubulin beta chain. Chain: b, d. Stathmin-4. Chain: e. Fragment: unp residues 49-189. Synonym: stathmin-like protein b3, rb3. Engineered: yes

Source:

Ovis aries. Sheep. Organism_taxid: 9940. Organ: brain. Rattus norvegicus. Rat. Organism_taxid: 10116. Gene: stmn4. Expressed in: escherichia coli.

Resolution:

4.00Å R-factor: 0.225 R-free: 0.250

Authors:

P.Barbier,A.Dorleans,F.Devred,L.Sanz,D.Allegro,C.Alfonso,M.Knossow, V.Peyrot,J.M.Andreu

Key ref:

P.Barbier et al. (2010). Stathmin and interfacial microtubule inhibitors recognize a naturally curved conformation of tubulin dimers. J Biol Chem, 285, 31672-31681. PubMed id: 20675373

Date:

18-May-10 Release date: 28-Jul-10

Protein chains

D0VWZ0  (D0VWZ0_SHEEP) -  Tubulin alpha chain from Ovis aries

Seq: 451 a.a.

Struc: 428 a.a.

Protein chains

D0VWY9  (D0VWY9_SHEEP) -  Tubulin beta chain from Ovis aries

Seq: 445 a.a.

Struc: 420 a.a.

Protein chain

P63043  (STMN4_RAT) -  Stathmin-4 from Rattus norvegicus

Seq: 189 a.a.

Struc: 123 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

J Biol Chem 285:31672-31681 (2010)

PubMed id: 20675373

Stathmin and interfacial microtubule inhibitors recognize a naturally curved conformation of tubulin dimers.

P.Barbier, A.Dorléans, F.Devred, L.Sanz, D.Allegro, C.Alfonso, M.Knossow, V.Peyrot, J.M.Andreu.

ABSTRACT

No abstract given.