PDBsum entry 3mxm

Hydrolase/DNA

PDB id: 3mxm

Contents

Protein chains

223 a.a.

DNA/RNA

Metals

IOD ×12

_CA ×4

Waters ×539

PDB id:

3mxm

Name:

Hydrolase/DNA

Title:

Trex1 3' exonuclease v201d aicardi-goutieres syndrome mutant

Structure:

Three prime repair exonuclease 1. Chain: b, a. Fragment: n-terminal fragment, residues 1-242. Synonym: 3'-5' exonuclease trex1. Engineered: yes. Mutation: yes. DNA (5'-d( Gp Ap Cp G)-3'). Chain: c, d. Engineered: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: trex1. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes

Resolution:

1.75Å R-factor: 0.173 R-free: 0.203

Authors:

S.L.Bailey,T.Hollis

Key ref:

S.L.Bailey et al. X-Ray crystal structures of trex1 3' Exonuclease auto disease mutants. To be published, .

Date:

07-May-10 Release date: 18-May-11

Protein chains

Q91XB0  (TREX1_MOUSE) -  Three-prime repair exonuclease 1 from Mus musculus

Seq: 314 a.a.

Struc: 223 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

G-A-C-G 4 bases

G-A-C-G 4 bases

Enzyme reactions

• Enzyme class: E.C.3.1.11.2 - exodeoxyribonuclease Iii.
• Reaction: Degradation of double-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.