PDBsum entry 3l2k

Biosynthetic protein

PDB id: 3l2k

Contents

Protein chains

352 a.a. *

Ligands

PXC ×2

Waters ×16

* Residue conservation analysis

PDB id:

3l2k

Name:

Biosynthetic protein

Title:

Structure of phenazine antibiotic biosynthesis protein with substrate

Structure:

Ehpf. Chain: a, b. Engineered: yes

Source:

Pantoea agglomerans. Erwinia herbicola. Organism_taxid: 549. Gene: ehpf. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.80Å R-factor: 0.216 R-free: 0.288

Authors:

A.K.Bera,V.Atanasova,J.F.Parsons

Key ref:

A.K.Bera et al. (2010). Structure of the D-alanylgriseoluteic acid biosynthetic protein EhpF, an atypical member of the ANL superfamily of adenylating enzymes. Acta Crystallogr D Biol Crystallogr, 66, 664-672. PubMed id: 20516619

Date:

15-Dec-09 Release date: 26-May-10

Protein chains

Q8GPH0  (Q8GPH0_ENTAG) -  EhpF from Enterobacter agglomerans

Seq: 366 a.a.

Struc: 352 a.a.

Acta Crystallogr D Biol Crystallogr 66:664-672 (2010)

PubMed id: 20516619

Structure of the D-alanylgriseoluteic acid biosynthetic protein EhpF, an atypical member of the ANL superfamily of adenylating enzymes.

A.K.Bera, V.Atanasova, S.Gamage, H.Robinson, J.F.Parsons.

ABSTRACT

The structure of EhpF, a 41 kDa protein that functions in the biosynthetic pathway leading to the broad-spectrum antimicrobial compound D-alanylgriseoluteic acid (AGA), is reported. A cluster of approximately 16 genes, including ehpF, located on a 200 kbp plasmid native to certain strains of Pantoea agglomerans encodes the proteins that are required for the conversion of chorismic acid to AGA. Phenazine-1,6-dicarboxylate has been identified as an intermediate in AGA biosynthesis and deletion of ehpF results in accumulation of this compound in vivo. The crystallographic data presented here reveal that EhpF is an atypical member of the acyl-CoA synthase or ANL superfamily of adenylating enzymes. These enzymes typically catalyze two-step reactions involving adenylation of a carboxylate substrate followed by transfer of the substrate from AMP to coenzyme A or another phosphopantetheine. EhpF is distinguished by the absence of the C-terminal domain that is characteristic of enzymes from this family and is involved in phosphopantetheine binding and in the second half of the canonical two-step reaction that is typically observed. Based on the structure of EhpF and a bioinformatic analysis, it is proposed that EhpF and EhpG convert phenazine-1,6-dicarboxylate to 6-formylphenazine-1-carboxylate via an adenylyl intermediate.