PDBsum entry 2f7f

Transferase

PDB id: 2f7f

Contents

Protein chain

482 a.a. *

Ligands

SO4 ×8

NIO ×2

DPO

GOL

Waters ×390

* Residue conservation analysis

PDB id:

2f7f

Name:

Transferase

Title:

Crystal structure of enterococcus faecalis putative nicotinate phosphoribosyltransferase, new york structural genomics consortium

Structure:

Nicotinate phosphoribosyltransferase, putative. Chain: a. Engineered: yes

Source:

Enterococcus faecalis. Organism_taxid: 226185. Strain: v583. Gene: ef2626. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Biol. unit:

Tetramer (from PDB file)

Resolution:

2.00Å R-factor: 0.172 R-free: 0.214

Authors:

Y.Patskovsky,S.C.Almo,S.K.Burley,New York Sgx Research Center For Structural Genomics (Nysgxrc)

Key ref:

Y.Patskovsky and s.c.almo Crystal structure of enterococcus faecalis nicotinate phosphoribosyltransferase. To be published, .

Date:

30-Nov-05 Release date: 13-Dec-05

Protein chain

Q830Y8  (Q830Y8_ENTFA) -  Nicotinate phosphoribosyltransferase from Enterococcus faecalis (strain ATCC 700802 / V583)

Seq: 494 a.a.

Struc: 482 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.6.3.4.21 - nicotinate phosphoribosyltransferase.
• Reaction: nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate

nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O (Bound ligand (Het Group name = NIO) corresponds exactly) = nicotinate beta-D-ribonucleotide + ADP (Bound ligand (Het Group name = DPO) corresponds exactly) + phosphate + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site