PDBsum entry 1vb6

Signaling protein

PDB id: 1vb6

Contents

Protein chains

114 a.a. *

Ligands

HEM

HEM-OXY

Waters ×315

* Residue conservation analysis

PDB id:

1vb6

Name:

Signaling protein

Title:

Crystal structure of the heme pas sensor domain of ec dos (oxygen- bound form)

Structure:

Heme pas sensor protein. Chain: a, b. Fragment: heme pas domain. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 83333. Strain: k12. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Biol. unit:

Dimer (from PQS)

Resolution:

1.56Å R-factor: 0.214 R-free: 0.232

Authors:

H.Kurokawa,M.Watanabe,I.Sagami,B.Mikami,T.Shimizu

Key ref:

H.Kurokawa et al. Structure of oxygen-Bound form of a heme pas domain of EC dos. To be published, .

Date:

24-Feb-04 Release date: 19-Apr-05

Protein chains

P76129  (DOSP_ECOLI) -  Oxygen sensor protein DosP from Escherichia coli (strain K12)

Seq: 799 a.a.

Struc: 114 a.a.

Enzyme reactions

• Enzyme class: E.C.3.1.4.52 - cyclic-guanylate-specific phosphodiesterase.
• Reaction: 3',3'-c-di-GMP + H2O = 5'-phosphoguanylyl(3'->5')guanosine + H⁺
• Cofactor: Heme; Mg(2+); Mn(2+)

Heme (Bound ligand (Het Group name = HEM) matches with 95.45% similarity) Mg(2+) Mn(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site