PDBsum entry 1v71

Isomerase

PDB id: 1v71

Contents

Protein chain

318 a.a. *

Ligands

PLP

Metals

_MG

Waters ×256

* Residue conservation analysis

PDB id:

1v71

Name:

Isomerase

Title:

Crystal structure of s.Pombe serine racemase

Structure:

Hypothetical protein c320.14 in chromosome iii. Chain: a. Synonym: serine racemase. Engineered: yes

Source:

Schizosaccharomyces pombe. Fission yeast. Organism_taxid: 4896. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Biol. unit:

Dimer (from PDB file)

Resolution:

1.70Å R-factor: 0.220 R-free: 0.249

Authors:

M.Goto

Key ref:

M.Goto Crystal structure of s.Pombe serine racemase. To be published, .

Date:

09-Dec-03 Release date: 14-Jun-05

Protein chain

O59791  (SRR_SCHPO) -  Serine racemase from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

Seq: 323 a.a.

Struc: 318 a.a.

Enzyme reactions

• Enzyme class 1: E.C.4.3.1.17 - L-serine ammonia-lyase.
• Reaction: L-serine = pyruvate + NH4⁺
• Cofactor: Pyridoxal 5'-phosphate or iron-sulfur

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = PLP) matches with 93.75% similarity) or iron-sulfur

• Enzyme class 2: E.C.4.3.1.18 - D-serine ammonia-lyase.
• Reaction: D-serine = pyruvate + NH4⁺
• Cofactor: Pyridoxal 5'-phosphate

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = PLP) matches with 93.75% similarity)

• Enzyme class 3: E.C.5.1.1.18 - serine racemase.
• Reaction: L-serine = D-serine
• Cofactor: Pyridoxal 5'-phosphate

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = PLP) matches with 93.75% similarity)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site