EC 4.3.1.17 - L-serine ammonia-lyase

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IntEnz Enzyme Nomenclature
EC 4.3.1.17

Names

Accepted name:
L-serine ammonia-lyase
Other names:
L-hydroxyaminoacid dehydratase
L-serine deaminase
L-serine dehydratase
L-serine hydro-lyase (deaminating)
serine deaminase
Systematic name:
L-serine ammonia-lyase (pyruvate-forming)

Reactions

Cofactor

Comments:

Most enzymes that catalyse this reaction are pyridoxal-phosphate-dependent, although some enzymes contain an iron-sulfur cluster instead [6]. The reaction catalysed by both types of enzymes involves the initial elimination of water to form an enamine intermediate (hence the enzyme's original classification as EC 4.2.1.13, L-serine dehydratase), followed by tautomerization to an imine form and hydrolysis of the C-N bond. The latter reaction, which can occur spontaneously, is also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase. This reaction is also carried out by EC 4.3.1.19, threonine ammonia-lyase, from a number of sources.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00149
Structural data: CSA , EC2PDB
Gene Ontology: GO:0003941
UniProtKB/Swiss-Prot: (47) [show] [UniProt]

References

  1. Ramos, F. and Wiame, J.-M.
    Occurrence of a catabolic L-serine (L-threonine) deaminase in Saccharomyces cerevisiae.
    Eur. J. Biochem. 123: 571-576 (1982). [PMID: 7042346]
  2. Simon, D., Hoshino, J. and Kröger, H.
    L-Serine dehydratase from rat liver. Purification and some properties.
    Biochim. Biophys. Acta 321: 361-368 (1973). [PMID: 4750769]
  3. Suda, M. and Nakagawa, H.
    L-Serine dehydratase (rat liver).
    Methods Enzymol. 17B: 346-351 (1971).
  4. Sagers, R.D. and Carter, J.
    E. L-Serine dehydratase (Clostridium acidiurica).
    Methods Enzymol. 17B: 351-566 (1971).
  5. Robinson, W.G. and Labow, R.
    L-Serine dehydratase (Escherichia coli).
    Methods Enzymol. 17B: 356-360 (1971).
  6. Grabowski, R., Hofmeister, A. E., Buckel, W.
    Bacterial L-serine dehydratases: a new family of enzymes containing iron-sulfur clusters.
    Trends Biochem. Sci. 18: 297-300 (1993). [PMID: 8236444]
  7. Yamada, T., Komoto, J., Takata, Y., Ogawa, H., Pitot, H. C., Takusagawa, F.
    Crystal structure of serine dehydratase from rat liver.
    Biochemistry 42: 12854-12865 (2003). [PMID: 14596599]

[EC 4.3.1.17 created 1961 as EC 4.2.1.13, transfered 2001 to EC 4.3.1.17, modified 2014]