PDBsum entry 1qqm

Hydrolase

PDB id: 1qqm

Contents

Protein chain

378 a.a. *

Ligands

PO4

ADP

Metals

_MG

__K

_CL ×2

Waters ×440

* Residue conservation analysis

PDB id:

1qqm

Name:

Hydrolase

Title:

D199s mutant of bovine 70 kilodalton heat shock protein

Structure:

D199s mutant of bovine 70 kilodalton heat shock protein. Chain: a. Fragment: hsc70 atpase fragment. Engineered: yes. Mutation: yes

Source:

Bos taurus. Cattle. Organism_taxid: 9913. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.90Å R-factor: 0.193 R-free: 0.234

Authors:

E.R.Johnson,D.B.Mckay

Key ref:

E.R.Johnson and D.B.McKay (1999). Mapping the role of active site residues for transducing an ATP-induced conformational change in the bovine 70-kDa heat shock cognate protein. Biochemistry, 38, 10823-10830. PubMed id: 10451379 DOI: 10.1021/bi990816g

Date:

07-Jun-99 Release date: 15-Sep-99

Protein chain

P19120  (HSP7C_BOVIN) -  Heat shock cognate 71 kDa protein from Bos taurus

Seq: 650 a.a.

Struc: 378 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.6.4.10 - non-chaperonin molecular chaperone ATPase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

ATP + H2O = ADP (Bound ligand (Het Group name = ADP) corresponds exactly) + phosphate + H(+) (Bound ligand (Het Group name = PO4) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1021/bi990816g

Biochemistry 38:10823-10830 (1999)

PubMed id: 10451379

Mapping the role of active site residues for transducing an ATP-induced conformational change in the bovine 70-kDa heat shock cognate protein.

E.R.Johnson, D.B.McKay.

ABSTRACT

ATP binding induces a conformational change in 70-kDa heat shock proteins (Hsp70s) that facilitates release of bound polypeptides. Using the bovine heat shock cognate protein (Hsc70) as a representative of the Hsp70 family, we have characterized the effect of mutations on the coupling between ATP binding and the nucleotide-induced conformational change. Steady-state solution small-angle X-ray scattering and kinetic fluorescence measurements on a 60-kDa fragment of Hsc70 show that point mutations K71M, E175S, D199S, and D206S in the nucleotide binding cleft impair the ability of ATP to induce a conformational change. A secondary mutation in the peptide binding domain, E543K, "rescues" the ATP-induced transition for three of these mutations (E175S/E543K, D199S/E543K, and D206S/E543K) but not for K71M/E543K. Analysis of kinetics of the ATPase cycle confirm that these effects do not result from unexpectedly rapid ATP hydrolysis or slow ATP binding. Crystallographic structures of E175S, D199S, and D206S mutant ATPase fragment proteins show that the mutations do not perturb the tertiary structure of the protein but do significantly alter the protein-ligand interactions, due in part to an apparent charge compensation effect whereby mutating a (probably) negatively charged carboxyl group to a neutral serine displaces a K+ ion from the nucleotide binding cleft in two out of three cases (E175S and D199S but not D206S).