PDBsum entry 1i2b

Biosynthetic protein

PDB id: 1i2b

Contents

Protein chain

393 a.a. *

Ligands

SO4 ×2

NAD

UPG

USQ

Waters ×402

* Residue conservation analysis

PDB id:

1i2b

Name:

Biosynthetic protein

Title:

Crystal structure of mutant t145a sqd1 protein complex with NAD and udp-sulfoquinovose/udp-glucose

Structure:

Sulfolipid biosynthesis protein sqd1. Chain: a. Engineered: yes. Mutation: yes

Source:

Arabidopsis thaliana. Thale cress. Organism_taxid: 3702. Strain: columbia (col-2). Gene: sqd1. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Dimer (from PDB file)

Resolution:

1.75Å R-factor: 0.180 R-free: 0.196

Authors:

M.J.Theisen,S.L.Sanda,S.L.Ginell,C.Benning,R.M.Garavito

Key ref:

M.J.Theisen et al. Characterization of the active site of udp-Sulfoquinovose synthase: formation of the sulfonic acid product in the crystalline state. To be published, .

Date:

07-Feb-01 Release date: 01-Jul-03

Protein chain

O48917  (SQD1_ARATH) -  UDP-sulfoquinovose synthase, chloroplastic from Arabidopsis thaliana

Seq: 477 a.a.

Struc: 393 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.13.1.1 - UDP-sulfoquinovose synthase.
• Pathway: UDP-glucose, UDP-galactose and UDP-glucuronate Biosynthesis
• Reaction: sulfite + UDP-alpha-D-glucose + H⁺ = UDP-alpha-D-6-sulfoquinovose + H2O

sulfite + UDP-alpha-D-glucose (Bound ligand (Het Group name = SO4) matches with 80.00% similarity) + H(+) (Bound ligand (Het Group name = UPG) corresponds exactly) = UDP-alpha-D-6-sulfoquinovose + H2O

• Cofactor: NAD(+)

NAD(+) (Bound ligand (Het Group name = NAD) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site