Dbfetch
ID HC476825; SV 1; linear; unassigned DNA; PAT; UNC; 12287 BP. XX AC HC476825; XX DT 21-APR-2010 (Rel. 104, Created) DT 21-APR-2010 (Rel. 104, Last updated, Version 1) XX DE Sequence 1 from Patent EP2166103. XX KW . XX OS unidentified OC unclassified sequences. XX RN [1] RA Song H.S., Kock M., Brown J.A., Loyall L.P., Xing L., Jia H., McMillan J., RA Ireland L.; RT "Method for isolation of transcription termination sequences"; RL Patent number EP2166103-A1/1, 24-MAR-2010. RL BASF Plant Science GmbH (DE). XX DR MD5; fc5df6c90876af93db08146b0ba2588d. XX FH Key Location/Qualifiers FH FT source 1..12287 FT /organism="unidentified" FT /mol_type="unassigned DNA" FT /note="Binary expression vector FT Lo546b-pSUN1-R4-Lo484::Lo376::Lo522b" FT /db_xref="taxon:32644" FT misc_feature 1..21 FT /note="attB4 recombination site" FT regulatory 49..905 FT /note="CaMV 35S" FT /regulatory_class="promoter" FT misc_feature 912..1719 FT /note="coding for mGFP5er" FT regulatory 1762..2431 FT /note="E9 terminator" FT /regulatory_class="terminator" FT misc_feature 2432..2455 FT /note="attB1 recombination site" FT misc_feature 2542..2565 FT /note="attb2 recombination site" FT regulatory 2581..3899 FT /note="STPT promoter" FT /regulatory_class="promoter" FT misc_feature 3930..4777 FT /note="coding for nptII" FT regulatory 4816..5494 FT /note="E9 terminator" FT /regulatory_class="terminator" FT regulatory 5500..5752 FT /note="complement: nos terminator" FT /regulatory_class="terminator" FT regulatory 5907..6169 FT /note="nos terminator" FT /regulatory_class="terminator" FT misc_feature 6283..6303 FT /note="complement: attB3 recombination site" FT rep_origin 8245..9352 FT /note="complement: ColE1" FT misc_feature 8245..9352 FT /note="repA" FT misc_feature 11116..11907 FT /note="aadA marker (Spectomycin)" FT misc_feature 12081..12226 FT /note="RB" XX SQ Sequence 12287 BP; 3348 A; 2981 C; 2939 G; 3019 T; 0 other; caactttgta tagaaaagtt ggccatgatt acgccaagct tgcatgcctg caggtcccca 60 gattagcctt ttcaatttca gaaagaatgc taacccacag atggttagag aggcttacgc 120 agcaggtctc atcaagacga tctacccgag caataatctc caggaaatca aataccttcc 180 caagaaggtt aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa 240 agatatattt ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca 300 caaaccaagg caagtaatag agattggagt ctctaaaaag gtagttccca ctgaatcaaa 360 ggccatggag tcaaagattc aaatagagga cctaacagaa ctcgccgtaa agactggcga 420 acagttcata cagagtctct tacgactcaa tgacaagaag aaaatcttcg tcaacatggt 480 ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag 540 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc 600 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca 660 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga 720 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa 780 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc 840 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagaa cacgggggac 900 tctagaggat ccaaggagat ataacaatga agactaatct ttttctcttt ctcatctttt 960 cacttctcct atcattatcc tcggccgaat tcagtaaagg agaagaactt ttcactggag 1020 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg 1080 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg 1140 gaaaactacc tgttccatgg ccaacacttg tcactacttt ctcttatggt gttcaatgct 1200 tttcaagata cccagatcat atgaagcggc acgacttctt caagagcgcc atgcctgagg 1260 gatacgtgca ggagaggacc atcttcttca aggacgacgg gaactacaag acacgtgctg 1320 aagtcaagtt tgagggagac accctcgtca acaggatcga gcttaaggga atcgatttca 1380 aggaggacgg aaacatcctc ggccacaagt tggaatacaa ctacaactcc cacaacgtat 1440 acatcatggc cgacaagcaa aagaacggca tcaaagccaa cttcaagacc cgccacaaca 1500 tcgaagacgg cggcgtgcaa ctcgctgatc attatcaaca aaatactcca attggcgatg 1560 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc 1620 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac 1680 atggcatgga tgaactatac aaacatgatg agctttaaga gaacggatcc ccatctgcgg 1740 ccgcctcgag catatgctag aggatcctct agctagagct ttcgttcgta tcatcggttt 1800 cgacaacgtt cgtcaagttc aatgcatcag tttcattgcg cacacaccag aatcctactg 1860 agtttgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa 1920 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa 1980 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct 2040 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt 2100 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacacttg 2160 tagttgtacc attatgctta ttcactaggc aacaaatata ttttcagacc tagaaaagct 2220 gcaaatgtta ctgaatacaa gtatgtcctc ttgtgtttta gacatttatg aactttcctt 2280 tatgtaattt tccagaatcc ttgtcagatt ctaatcattg ctttataatt atagttatac 2340 tcatggattt gtagttgagt atgaaaatat tttttaatgc attttatgac ttgccaattg 2400 attgacaaca tgcatcaatc gaccgggtac ccaagtttgt acaaaaaagc aggctggtac 2460 ccggggatcc tctaggtcga ccagatctga tatctgcggc cgcctcgagc atatgggcat 2520 gcaagcttgg cgtaatcatg gacccagctt tcttgtacaa agtggggtac ccggggatcc 2580 tgatagctta tactcaaatt caacaagtta tatataaatg tatagatact acaatatcat 2640 taacaaaagt caccttaaat aaatacacat atcttttatg ttctctattg ttttgcgtac 2700 gctaacacaa tttctcatat gcaaaaggat gaatgagtaa caaattacct cataagaaca 2760 atcatctttg cttacatact aatacaataa tcactcaatc aaccaataac atcaatcaca 2820 taggtttaca tacaataatc actcaatcaa cttcataaga agaatcatgt ttacttaatt 2880 catcaattat ccccaaaaac accactatta agtataaact acaacatatt tgtagtgatg 2940 ggtcaacatt tttatcatat ttaaactcgg gttccctcaa atcgagaaat agtgaacatg 3000 taatattaat tttaaatcgc aattacagaa attaattgaa tttggtcaaa tggacagaat 3060 tttatagatt gggtggaact agaaaaaaaa aaaaaaagag tatagggtga attgagtaca 3120 tgaaagtaca tggtaatcct agttaaacgc ataatacatg tgggttcatt tgtatttttt 3180 tgtaacttac gagtaaactg gctacaacaa aaaaaaatta gaagattttt ttgttttgta 3240 gaaaacccta attttagtta tagttgtata actttgataa aattataaaa ttgtattacg 3300 aaaaaagtaa taagatattc aaaaaagcct agaataacgt atatgactat gagcatgaaa 3360 ctgcaagtca aatgctgaca gacaaccata aacaaaagaa attaaataga gataccttta 3420 aaataagtaa aatttcattt ataaaaaatc tactttcttg tgaatctgtc acgttcaata 3480 atttgaagac cactcaacat acaaggtaaa taatgaaaaa taaaatctac caaaatttca 3540 atcattatta tcttccaaaa aaacaaaatt atacagatga tgatggtgat atggaacttc 3600 gattggctaa tattcactgt gtctctaaaa accatccact tatcaagata agatggaccc 3660 tacactcatc caatctaaac cagtatctca agattcttat ctaattacat cattctctac 3720 cgttagatga aattgaccat taaccctacc ataactccat acaccgcgag atactggatt 3780 aaccaaatcg agatcatcgt agccgtccga tcaacaagta ccatctcttg aaatactcga 3840 aatcctcata agtccgtccc tctttgctct cactatcaaa actctgaatt tcgatttcat 3900 ctagagtcga gcccgggcga tatcggatct cgactctagt cgagggccca tgggagcttg 3960 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 4020 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 4080 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 4140 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 4200 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 4260 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 4320 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 4380 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 4440 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 4500 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 4560 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 4620 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 4680 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 4740 cgagttcttc tgagcgggac ccaagctagc ttcgacggat cctctagcat atgctcgagg 4800 cggccgcaga tatcagatcc tctagctaga gctttcgttc gtatcatcgg tttcgacaac 4860 gttcgtcaag ttcaatgcat cagtttcatt gcgcacacac cagaatccta ctgagtttga 4920 gtattatggc attgggaaaa ctgtttttct tgtaccattt gttgtgcttg taatttactg 4980 tgttttttat tcggttttcg ctatcgaact gtgaaatgga aatggatgga gaagagttaa 5040 tgaatgatat ggtccttttg ttcattctca aattaatatt atttgttttt tctcttattt 5100 gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca ttttgttttg agtaaaaatg 5160 tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg agtaaaacac ttgtagttgt 5220 accattatgc ttattcacta ggcaacaaat atattttcag acctagaaaa gctgcaaatg 5280 ttactgaata caagtatgtc ctcttgtgtt ttagacattt atgaactttc ctttatgtaa 5340 ttttccagaa tccttgtcag attctaatca ttgctttata attatagtta tactcatgga 5400 tttgtagttg agtatgaaaa tattttttaa tgcattttat gacttgccaa ttgattgaca 5460 acatgcatca atcgacctgc agcccaagct tgatctagtc cgatctagta acatagatga 5520 caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta 5580 aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta 5640 catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag 5700 accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatcggggaa 5760 attcgagctc ggtagcaatt cccgaggctg tagccgacga tggtgcgcca ggagagttgt 5820 tgattcattg tttgcctccc tgctgcggtt tttcaccgaa gttcatgcca gtccagcgtt 5880 tttgcagcag aaaagccgcc gacttgtcga ccgatcggtc aaacatttgg caataaagtt 5940 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt 6000 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta 6060 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa 6120 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa gcttggcgta 6180 atcatggcaa ctttattata caaagttggc attataaaaa agcattgctt atcaatttgt 6240 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta ttcaacttta ttatacatag 6300 ttgataattc actggccgga tctgcttggt aataattgtc attagattgt ttttatgcat 6360 agatgcactc gaaatcagcc aattttagac aagtatcaaa cggatgttaa ttcagtacat 6420 taaagacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata 6480 tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccacg 6540 cgttaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt 6600 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg 6660 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct 6720 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc 6780 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg 6840 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga 6900 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt 6960 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac 7020 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc 7080 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta 7140 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa 7200 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag 7260 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc 7320 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc 7380 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg 7440 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg 7500 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg 7560 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac 7620 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg 7680 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct 7740 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg 7800 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg 7860 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag 7920 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt 7980 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac 8040 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat 8100 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag 8160 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg 8220 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt 8280 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt 8340 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc 8400 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc 8460 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt 8520 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt 8580 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg 8640 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt 8700 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga 8760 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc 8820 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca 8880 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg 8940 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat 9000 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt 9060 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg 9120 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga 9180 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg 9240 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct 9300 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga 9360 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt 9420 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg 9480 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa 9540 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct 9600 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct 9660 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg 9720 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc 9780 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg 9840 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 9900 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 9960 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 10020 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 10080 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 10140 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 10200 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 10260 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 10320 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 10380 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 10440 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 10500 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 10560 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 10620 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 10680 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 10740 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 10800 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 10860 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 10920 ctcacgttaa gggattttgg tcatgcatga tatatctccc aatttgtgta gggcttatta 10980 tgcacgctta aaaataataa aagcagactt gacctgatag tttggctgtg agcaattatg 11040 tgcttagtgc atctaacgct tgagttaagc cgcgccgcga agcggcgtcg gcttgaacga 11100 atttctagct agacattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac 11160 aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc 11220 ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc 11280 ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa 11340 cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa 11400 ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc 11460 cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc 11520 aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc 11580 aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat 11640 ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag 11700 gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa 11760 atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc 11820 cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga 11880 tacttcggcg atcaccgctt cccccatgat gtttaacttt gttttagggc gactgccctg 11940 ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg 12000 ctgcttggat gcccgaggca tagactgtac cccaaaaaaa cagtcataac aagccatgaa 12060 aaccgccact gcgttccatg gacatacaaa tggacgaacg gataaacctt ttcacgccct 12120 tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat 12180 cctgtcaaac actgatagtt taaactgaag gcgggaaacg acaatcagat ctagtaggaa 12240 acagctatga ccatgattac gccaagctat cgattacgcc aagctat 12287 //