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- What are volume data?
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Volume matching use case
Aligning bacterial ribosomes
The ribosome is a large and complex molecular machine that serves as the site of biological protein synthesis. The bacterial ribosome is considered to consist of three rRNA molecules and around 50 proteins. However, it is also the site for a large number of additional proteins that have specific roles in the life cycle of the ribosome. Aligning volume data for ribosomes can help to identify these additional proteins, and any structural changes they may trigger.
Tetracycline resistance protein TetO protects the bacterial ribosome from binding the antibiotic tetracycline. It is a translational GTPase with significant similarity to the elongation factor EF-G. We can compare the 9.8 Å volume of a TetO-bound bacterial ribosome (EMD-5562) with a 9.9 Å volume of a bacterial ribosome with EF-G (and RRF) bound (EMD-1917).