Methods in proteomics

Several high-throughput technologies have been developed to investigate proteomes in depth. The most commonly applied are mass spectrometry (MS)-based techniques such as Tandem-MS and gel-based techniques such as differential in-gel electrophoresis (DIGE). These high-throughput technologies generate huge amounts of data. Databases are critical for recording and carefully storing this data, allowing the researcher to make connections between their results and existing knowledge. 

The EBI hosts up-to-date and accurate databases to enable rapid searching and retrieval of these data. The four major databases related to proteomic research (UniProtKBIntActReactome and PRIDE) are described in the next section. These four databases (especially UniProtKB) draw from gene sequence data (e.g. Ensembl) and annotation tools (e.g. InterPro) also hosted by the EBI. You can find out more about such resources in other training courses, such as the UniProt quick tour and the InterPro quick tour.