- Course overview
- Search within this course
- What is Pfam?
- Searching a specific Pfam entry
- Searching Pfam by domain architecture
- Getting Pfam data
- Your feedback
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Searching Pfam using a protein sequence
Searching Pfam with a protein sequence
One of the most valuable uses of IntePro is to find which entry (or entries) a sequence matches, and thus what its potential function might be. To run a search you can follow this steps, all them highlighted in Figure 8:
- Click on Search By Sequence in the InterPro website menu.
- Provide the FASTA formatted sequence(s) (protein or nucleotide) of interest by pasting them into the test box or by importing them from a fasta file. Up to 100 sequences can be submitted.
- Expand the Advanced options, click on Unselect all protein sequence applications, and select Pfam.
- Click on the Search button.
An example set of results is shown on the next page.