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Mass spectrometry-based proteomics
Mass spectrometry-based proteomics has revolutionised the analysis of proteins by enabling quantitative analysis of thousands of proteins and their properties, including post-translational modification (PTM). The resulting datasets are rich, multidimensional, and comprehensive.
This webinar with Tony Ly (Sir Henry Dale Fellow, University of Dundee), recorded on 16 March 2022, provides basics of peptide and PTM identification by mass spectrometry, strategies for quantitation, and introduce tools for the analysis of proteomic datasets.
By the end of the webinar recording you will be able to:
- Describe the basics of peptide identification by mass spectrometry
- Explain the principles of protein/PTM quantitation, e.g. isobaric labels (TMT), DDA, DIA
- Identify the tools available for PTM and sequence logo analysis
You may either watch the entire presentation from the introduction or navigate directly to a specific section by clicking the links provided below:
- Introduction
- Peptide identification for large scale proteomics
- What is a good peptide score?
- Quantitative approaches in Mass Spectrometry-based proteomics
- Label-free quantitation
- Isobaric labeling
- Considerations for phosphoproteomics
- Tools for motif enrichment
- Q&A
After learning about peptide identification and protein quantification, continue on to the next page where we will provide a deeper insight into the significance of protein interactions, and methods to identify and capture such interactions.