- Course overview
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- What is antimicrobial resistance?
- How do we study pathogens?
- Public pathogen data
- A guide to the Pathogens Portal
- Analysing genomic data from pathogens
- Looking for antimicrobial resistance genes in different environments
- Data sharing
- The future of AMR
- Crossword: Test your knowledge
- Your feedback
- Further resources
- Help and support
- Glossary
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ARG Analysis: Locating genes in Ensembl Bacteria
Pseudomonas aeruginosa UCBPP-PA14 (GCA_000014625.1) is a clinical isolate from a human burn patient. It is infectious in a range of organisms and is known to encode a number of antibiotic resistance genes. One gene of interest is FosA, a glutathione transferase enzyme which can inactivate the antibiotic fosfomycin.
Let’s investigate this gene further using Ensembl bacteria. Follow the steps below to complete the exercise!
1. Using Ensembl Bacteria (Release 60) search for the Pseudomonas aeruginosa UCBPP-PA14 genome.
2. Once on the strain page search for the gene FosA, and follow the link to the Gene ID page to explore further.
3. Click on the gene (highlighted in green) within the browser to access information about it.
Once you have worked through the steps have a go at answering the questions below.
Continue on to the next page to complete the second part of the activity.