Getting data from IntAct

Quick search

On the IntAct homepage use the search box to type in the protein, gene or chemical name of your molecule of interest, or a UniProtKB or ChEBI accession number, to see a binary view of all of the known interaction partners of that molecule (Figure 3). Drill down to see the details of any one piece of interaction evidence, such a mutations affecting its outcome, binding regions or kinetic parameters. View selected interactions in a simple graphical interface or export to Cytoscape for further analysis. Advanced search options are available for more complex queries.

Data download

The IntAct project co-develops and supports the PSI-MI standard (see information box) and provides both a web service and a simple URL-based interface that allow direct computational access to retrieve interaction networks in PSI-MI format. A simple, tab-delimited file is also provided to list all mutations affecting interactions as represented in IntAct. Additionally, all files can be accessed and downloaded from the FTP site for local installation (Figure 4).

What is the PSI-MI format? The PSI-MI format (Proteomics Standards Initiative Molecular Interaction format) is a community standard data model for representing and exchanging molecular interaction data. It has been jointly developed by members of the Proteomics Standards Initiative (PSI), a work group of the Human Proteome Organization (HUPO), and is supported by major protein interaction data providers.