How is the sequence annotated

Annotation is optionally submitted by the sequencing group when they submit the sequence or at a later stage. However, ENA will accept the submission of Third Party Annotation (TPA) from people other than the sequencing group as long as there is a published reference for the annotation. TPA is placed in a separate entry from the original submission (Figure 7).

There are two types of TPA datasets:
  • TPA:experimental data are supported by peer-reviewed experimental evidence.
  • TPA:inferential are supported by peer-reviewed indirect experimental evidence. For instance,  annotation inferred through homologous genes of experimentally-determined function.

ENA entry containing TPA: Experimental data      Figure 7. ENA entry containing TPA: Experimental data.


[A] Description identifies this entry as containing TPA data.

[B] Keywords, such as TPA, can be used in a text search.

[C] Assembly provides a link to the original sequence entry/entries.

[D] References to peer-reviewed journals are required for TPA annotation.


Ensembl is another source of genome annotation. Try the online course: Ensembl: Browsing chordate genomes.