Want to know how we did it?



 1. Perform a simple search putting the UniProtKB accession of VCP (P55072) in the search box in the IntAct website.

2. Once you have the table with the results (104 interactions as of March 2012), you could try to work out which proteins have been found to interact with VCP more than once in the table. However, it will be much easier to detect these using a graphical representation.  

3. Click on the Graph tab.

4. Once you get the network visualisation, look at the molecules that interact with VCP. The proteins are represented by balls and the small molecules are represented by triangles. Check different layouts until you are happy with one.

5. To visualise those partners that have been found to interact with VCP more than once, go to CytoscapeWeb Controls and deactivate the "Merge edges" feature.

6. Proteins such as UBXN-1, -6 and -7 and AMFR have been found to interact with VCP in multiple instances, as shown by the multiple edges that connect them with VCP. You can go back to the tabular view to learn more about each interaction if you want.