Submitting data to the ENA

Many journals and funders require authors to submit their sequence information to a database that is a member of the International Nucleotide Sequence Database Collaboration (INSDC; see information on collaborations in get help and support on the ENA) prior to publication. The advantage of submitting your sequence data to the ENA is that your data will be permanently available and readily accessible to scientists worldwide. After submission, accession numbers are assigned to identify your sequence and any related information. These accession numbers should be included in your manuscript. You can choose whether to make your data publicly available immediately or wait until your paper is published.

Manual submissions

You can register new sequencing projects and submit assembled sequence and annotation to the ENA using Webin, the EBI’s preferred web-based submission system. Webin provides interactive web forms that are tailored to the type of data to be submitted and that capture and validate the information required. Sequence and annotation can also be uploaded to Webin in several formats. Webin is available from the ENA's submissions login page.

To submit small-scale raw sequence data, send an e-mail request to datasubs [at] (submissions)

and a secure Webin-box will be set up. Users can upload data and metadata files (prepared in third party editors) into the Webin-box by FTP or Aspera and using a dedicated webpage.

Automated submissions

The EBI works closely with sequencing centres and other facilities to ensure the timely incorporation of data into the ENA. A number of options are available for automated submissions, including submission accounts for annotated sequences, the RESTful web-based submission service for next-generation sequence metadata, as well as FTP and Aspera drop-boxes. All enquiries should be directed to datasubs [at] (submissions).

Updating ENA content

Records can eventually become out of date; authors might need to make corrections to sequence and assemblies, or they might discover new features that need to be added through annotation. Because such findings are rarely published in journals, it is important that authors communicate their new findings to the ENA. Authors wishing to do so should use the update procedure available from the submissions page.