Visualising data from the Enzyme Portal

Enzyme summary tab

If you click on an enzyme name (or one of its orthologues) in the search results, you will be directed to the enzyme page, organised into tabs (on the left-hand side). The first is the enzyme summary tab, which contains the description of the enzyme function, its classification in the EC hierarchy, any synonyms and some information about the protein sequence (Figure 4):

Figure 4 Enzyme summary page for EC 3.6.3.49 (CFTCR)

Note the 'organisms' drop-down menu at the top. It can be used to switch between the known orthologues of the enzyme.

Throughout the portal, all data sources are acknowledged and linked, so that you can access in-depth information (such as the protein sequence from UniProt).

Protein structure tab

The protein structure tab shows any experimental 3D models of the enzyme (Figure 5). If there are several of them, you can select one from the drop-down menu, which indicates the number of structures available.

Figure 5 Protein structure tab for EC 3.6.3.49

Reactions and pathways tab

The reactions and pathways tab shows the biochemical reaction(s) catalysed by the enzyme and the chemical structures of the participants are linked to ChEBI (Figure 6). It also shows any metabolic pathways that the enzyme may participate in.

Figure 6 Reactions and pathways for EC 3.6.3.49

NB. Only the summary information is shown in this tab. For more information, you can click through to the data sources (in this case, Rhea and Reactome).

Small molecules tab

In the small molecules tab you can find cofactors, activators, inhibitors and drugs, as well as any bioactive compounds that are associated with the enzyme (Figure 7). You can also click on the chemical structures to navigate to ChEBI or ChEMBL.

Figure 7 Small molecules related to EC 3.6.3.49

Disease tab

The disease tab lists any diseases that are related to the selected enzyme, with a short description of the disease and how it affects the enzyme's function (Figure 8).

Figure 8 Disease related to EC 3.6.3.49

Literature tab

The literature tab lists bibliographic citations relevant to the enzyme (see Figure 9).

Article titles are linked to the EBI's CiteXplore bibliography database. If an abstract is available, you can view it by clicking on 'toggle abstract'.

Note the filters at the top of the list: these are related to the other tabs (enzyme summary, protein structure, reactions and pathways, small molecules, diseases). These filters allow you to view papers that are relevant to one of these enzyme characteristics.

Figure 9 Literature about EC 3.6.3.49