Part 1


In the first talk, Matthias introduces how we can assemble whole genomes from NGS data using the Velvet genome assembly programme. He discusses the different kinds of NGS reads that can be used. Following that is an explanation of how Velvet uses De Bruijn graphs to assemble genomes, including:

  • What De Bruijn graphs are.
  • How we can use them to assemble sequences.
  • How we can resolve bubbles in De Bruijn graphs using Tour Bus.
  • An example assembly process using De Bruijn graphs.
  • The benefits and difficulties of using De Bruijn graphs.

This talk includes an introduction to the first practical, as well as questions and answers from the practical. If you do not wish to hear the questions and answers about the practical, pause the video at 28m30s.