Technical help sheet

Technical requirements for data analysis

You will require the following tools for executing the practicals in this course:

CarveMehttps://github.com/cdanielmachado/carveme
SMETANAhttps://github.com/cdanielmachado/smetana
Snakemakehttps://github.com/snakemake/snakemake
Rhttps://www.r-project.org/
Tidyversehttps://github.com/tidyverse/tidyverse
ggAlluvialhttps://github.com/corybrunson/ggalluvial/
githttps://git-scm.com/
IBM CPLEX Optimizerhttps://www.ibm.com/uk-en/products/ilog-cplex-optimization-studio
pandashttps://github.com/pandas-dev/pandas
seabornhttps://seaborn.pydata.org/
matplotlibhttps://matplotlib.org/
framedhttps://github.com/cdanielmachado/framed
reframedhttps://github.com/cdanielmachado/reframed
randomhttps://docs.python.org/3/library/random.html
numpyhttps://numpy.org/
globhttps://docs.python.org/3/library/glob.html
scipyhttps://scipy.org/
jupyterhttps://jupyter.org/install
minicondahttps://docs.conda.io/en/latest/miniconda.html
ggpubrhttps://cran.r-project.org/web/packages/ggpubr/index.html
prodigalhttps://github.com/hyattpd/Prodigal
python3
minicondahttps://docs.conda.io/en/latest/miniconda.html
text editors (vim, emacs, nano, gedit, vscode)
curl, wget
jqhttps://stedolan.github.io/jq/
jupyterhttps://jupyter.org/install
dockerhttps://docs.docker.com/get-docker/
sourmash python packagehttps://sourmash.readthedocs.io/en/latest/
pandashttps://github.com/pandas-dev/pandas
metaSPAdeshttps://github.com/ablab/spades/releases/tag/v3.15.3
assembly_statshttps://github.com/MikeTrizna/assembly_stats/
Bandagehttps://rrwick.github.io/Bandage/
BLASThttps://www.ncbi.nlm.nih.gov/books/NBK569861/
megahithttps://github.com/voutcn/megahit
bwahttps://sourceforge.net/projects/bio-bwa/files/
samtoolshttps://sourceforge.net/projects/samtools/files/samtools/
MetaBAThttps://bitbucket.org/berkeleylab/metabat/downloads/
CheckMhttps://github.com/Ecogenomics/CheckM
newick utilshttps://github.com/tjunier/newick_utils
FigTreehttps://github.com/rambaut/figtree
Java Runtime Environment
fastqchttps://www.bioinformatics.babraham.ac.uk/projects/fastqc/
multiqchttps://github.com/ewels/MultiQC
bowtie2https://github.com/BenLangmead/bowtie2
biobakey-kneaddatahttps://github.com/biobakery/kneaddata
blast extecutables (blastn, makeblastdb)https://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Web&PAGE_TYPE=BlastDocs&DOC_TYPE=Download
kraken2https://github.com/DerrickWood/kraken2