Technical help sheet

Introductory computational and data visualisation skills

Rstudio & Rhttps://posit.co/products/open-source/rstudio/
R libraries: tidyverse, ggplot2, gProfiler2install.packages(c(“tidyverse”, “gprofiler2”)

Genome variation across human populations

Variant Effect Predictor (either the command line script or its docker image)https://www.ensembl.org/info/docs/tools/vep/index.html
Samtools v1.12http://www.htslib.org/download/
Bcftools v1.12http://www.htslib.org/download/
GATK v4.2.0.0https://github.com/broadinstitute/gatk/releases
BWAhttps://sourceforge.net/projects/bio-bwa/files/bwa-0.7.17.tar.bz2/download
IGV
(The web browser version should be fine)
https://igv.org/
VEPhttps://www.ensembl.org/info/docs/tools/vep/script/vep_tutorial.html
Data for VEPhttps://ftp.ensembl.org/pub/current_variation/indexed_vep_cache/
homo_sapiens_vep_109_GRCh38.tar.gz

Modelling cell signalling pathways

COPASIhttp://copasi.org/
Jupytor Notebook with Tellurium Package installedhttps://tellurium.readthedocs.io/en/latest/installation.html

Interpreting functional information from large scale protein structure data

PyMolhttps://pymol.org/2/
Jupyterhttps://jupyter.org/
CCP4https://www.ccp4.ac.uk/download/download_file.php?pkg=setup-64&os=linux&sid=ae2fafe0f387f669960cfa4fc9e7e85b0d570c7f
Python with packages: sys, pprint, solrq, pandas, numpy, requests, matplotlib, ipythonhttps://www.python.org/
VSCodehttps://code.visualstudio.com/sha/download?build=stable&os=linux-deb-x64

Networks and pathways

Cytoscape https://cytoscape.org/
Rstudiohttps://www.rstudio.com/
SummarizedExperiment R packagehttps://bioconductor.org/packages/release/bioc/html/SummarizedExperiment.html
limma R packagehttps://bioconductor.org/packages/release/bioc/html/limma.html
gprofiler2https://cran.r-project.org/web/packages/gprofiler2/index.html
tidyversehttps://www.tidyverse.org/