Modelling protein structure and missense variants: Phyre2 and Missense3D in the context of AlphaFold models

Trainer: Michael Sternberg, Harry Powell and Alessia David

Overview: This lecture first covers homology modelling theory and its use in the automatic modelling software Phyre2 for predicting macromolecular structure. Thereafter missense mutation prediction and modelling using Missense3D is covered.

Learning outcomes:

By the end of this lecture you should be able to:

  • Describe the theory behind template-based modelling of protein structures
  • Apply missense mutations to protein structures using Missense3D

Materials: