Technical help sheets

Technical requirements for data analysis

You will require the following tools for executing the practicals in this course:

Raw reads to Expression Matrix (EM)

CellRangerhttps://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/installation
SRA Toolkithttps://github.com/ncbi/sra-tools/wiki/02.-Installing-SRA-Toolkit
Fastqchttps://kb.10xgenomics.com/hc/en-us/articles/360048465032-When-to-run-FastQC-
TrimGalorehttps://github.com/FelixKrueger/TrimGalore
R, dplyrhttps://www.r-project.org/nosvn/pandoc/dplyr.html
R, cowplothttps://cran.r-project.org/web/packages/cowplot/readme/README.html
R, ggplot2https://ggplot2.tidyverse.org/
R, tibblehttps://cran.r-project.org/web/packages/tibble/readme/README.html
R, Matrixhttps://rdrr.io/rforge/Matrix/
R, readrhttps://cran.r-project.org/web/packages/readr/readme/README.html
R, pathworkhttps://cran.r-project.org/web/packages/patchwork/readme/README.html
R, tidyrhttps://www.r-project.org/nosvn/pandoc/tidyr.html
R, RColorBrewerhttps://rdrr.io/cran/RColorBrewer/
R, org.Hs.eg.dbhttps://bioconductor.org/packages/release/data/annotation/html/org.Hs.eg.db.html
R, glmGamPoihttps://bioconductor.org/packages/release/bioc/html/glmGamPoi.html
R, hdf5rhttps://github.com/hhoeflin/hdf5r
R, DoubletFinderhttps://github.com/chris-mcginnis-ucsf/DoubletFinder
Anacondahttps://www.anaconda.com/products/distribution#Downloads


Pre-precessing and Dimensionality Reduction / From UMAP to Annotation

R Studiohttps://www.rstudio.com/products/rstudio/download/
R, latest versionhttps://cran.r-project.org/
Covid data, sample C143https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM4339771
Covid data, sample C145https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM4339773
Covid data, sample C51https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM4475048
Covid data, sample C52https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM4475049
R package SingleCellExperimenthttps://bioconductor.org/packages/release/bioc/html/SingleCellExperiment.html
R package Matrixhttps://cran.r-project.org/web/packages/Matrix/index.html
R package DropletUtilshttps://bioconductor.org/packages/release/bioc/html/DropletUtils.html
R package scranhttps://bioconductor.org/packages/release/bioc/html/scran.html
R package batchelorhttps://www.bioconductor.org/packages/release/bioc/html/batchelor.html
R package Seurathttps://cran.r-project.org/web/packages/Seurat/index.html
R package uwothttps://github.com/jlmelville/uwot
R package dplyrhttps://cran.r-project.org/web/packages/dplyr/index.html
R package irlbahttps://cran.r-project.org/web/packages/irlba/index.html
R package miloR (development version)https://github.com/MarioniLab/miloR
R package stringrhttps://cran.r-project.org/web/packages/stringr/index.html
R package gdatahttps://cran.r-project.org/web/packages/gdata/index.html
R package DoubletFinderhttps://github.com/chris-mcginnis-ucsf/DoubletFinder
R package ggplot2https://ggplot2.tidyverse.org/
R package ggpubrhttps://cran.r-project.org/web/packages/ggpubr/index.html
R package MouseGastrulationDatahttps://bioconductor.org/packages/release/data/experiment/html/MouseGastrulationData.html
R package viridishttps://cran.r-project.org/web/packages/viridis/index.html
Magick++https://imagemagick.org/Magick++/
R package igraphhttps://cran.r-project.org/web/packages/igraph/index.html
R package celldexhttp://bioconductor.org/packages/release/data/experiment/html/celldex.html
R package SingleRhttps://bioconductor.org/packages/release/bioc/html/SingleR.html
R package pheatmaphttps://cran.r-project.org/web/packages/pheatmap/index.html
R package SC3https://www.bioconductor.org/packages/release/bioc/html/SC3.html
R package Harmonyhttps://cran.r-project.org/web/packages/harmony/index.html
R package Seurathttps://cran.r-project.org/web/packages/Seurat/index.html
R package Azimuthhttps://github.com/satijalab/azimuth
R package SeuratDatahttps://github.com/satijalab/seurat-data
R package patchworkhttps://cran.r-project.org/web/packages/patchwork/index.html
R package blusterhttps://bioconductor.org/packages/3.15/bioc/html/bluster.html
R package scaterhttps://bioconductor.org/packages/release/bioc/html/scater.html


Cell Atlases & Interpretation