{"id":273,"date":"2022-08-04T15:22:20","date_gmt":"2022-08-04T15:22:20","guid":{"rendered":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-r-handbook-2022\/programme\/day-1\/library-generation-overview\/"},"modified":"2026-03-10T10:48:14","modified_gmt":"2026-03-10T10:48:14","slug":"library-generation-overview","status":"publish","type":"page","link":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-materials\/planing-your-experiment\/library-generation-overview\/","title":{"rendered":"Wet-lab overview\u00a0"},"content":{"rendered":"\n<p><strong>Trainer:<\/strong> Marisa Loach, Kayleigh Smith, Wendi Bacon<\/p>\n\n\n\n<p><strong>Overview:<\/strong>&nbsp;This session will describe the steps in droplet-based scRNA seq data generation.<br><br>Why does this matter?<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>The analysis and troubleshooting in particular relies on understanding the library generation and cell isolation processes<\/li>\n\n\n\n<li>Also, kits! You&#8217;d be surprised how few highly experienced wet-labbers actually know what their kit is doing\u2026<\/li>\n<\/ul>\n\n\n\n<p><strong>Materials:<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><mark><span style=\"color:#000000\" class=\"tadv-color\"><span style=\"background-color:#ffffff\" class=\"tadv-background-color\"><a href=\"https:\/\/ftp.ebi.ac.uk\/pub\/training\/2026\/Single_cell_rna_seq_analysis_with_python_2026\/Presentations\/wet-lab_overview.pptx\">Presentation slides<\/a><\/span><\/span><\/mark><\/li>\n\n\n\n<li><a href=\"https:\/\/www.youtube.com\/watch?v=LD_c7PYIc7I\">Video recording on Explaining 10X Chromium<\/a><br><\/li>\n<\/ul>\n\n\n\n<p><strong>Droplet-based library generation activity<\/strong><\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Make a copy of this\u00a0<a href=\"https:\/\/ftp.ebi.ac.uk\/pub\/training\/2026\/Single_cell_rna_seq_analysis_with_python_2026\/Presentations\/Wet-lab_Activity\/Wet-lab_Activity.pptx\">activity template<\/a><\/li>\n\n\n\n<li>Put the labels &amp; images in order, from cells to sequencing<\/li>\n<\/ol>\n\n\n\n<figure class=\"wp-block-image is-style-default\"><img decoding=\"async\" src=\"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-materials\/wp-content\/uploads\/sites\/30\/2021\/07\/image.png\" alt=\"Machine generated alternative text:\n\n\" class=\"wp-image-138\"\/><\/figure>\n\n\n\n<p>3. Check\u00a0<a href=\"https:\/\/ftp.ebi.ac.uk\/pub\/training\/2026\/Single_cell_rna_seq_analysis_with_python_2026\/Presentations\/Wet-lab_Activity\/Wet-lab_Overview_Answers.pdf\">answer key<\/a>\u00a0for the results<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Trainer: Marisa Loach, Kayleigh Smith, Wendi Bacon Overview:&nbsp;This session will describe the steps in droplet-based scRNA seq data generation. Why does this matter? Materials: Droplet-based library generation activity 3. Check\u00a0answer key\u00a0for the results<\/p>\n","protected":false},"author":6,"featured_media":0,"parent":1937,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-273","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-materials\/wp-json\/wp\/v2\/pages\/273","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-materials\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-materials\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-materials\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-materials\/wp-json\/wp\/v2\/comments?post=273"}],"version-history":[{"count":21,"href":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-materials\/wp-json\/wp\/v2\/pages\/273\/revisions"}],"predecessor-version":[{"id":2401,"href":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-materials\/wp-json\/wp\/v2\/pages\/273\/revisions\/2401"}],"up":[{"embeddable":true,"href":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-materials\/wp-json\/wp\/v2\/pages\/1937"}],"wp:attachment":[{"href":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-materials\/wp-json\/wp\/v2\/media?parent=273"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}