{"id":273,"date":"2022-08-04T15:22:20","date_gmt":"2022-08-04T15:22:20","guid":{"rendered":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-r-handbook-2022\/programme\/day-1\/library-generation-overview\/"},"modified":"2025-02-17T15:28:25","modified_gmt":"2025-02-17T15:28:25","slug":"library-generation-overview","status":"publish","type":"page","link":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-handbook-2025\/programme-2\/day-1\/library-generation-overview\/","title":{"rendered":"Wet-lab overview\u00a0"},"content":{"rendered":"\n<p><strong>Monday 17 February 2025<\/strong><\/p>\n\n\n\n<p><strong>Trainer:<\/strong> Wendi Bacon<\/p>\n\n\n\n<p><strong>Overview:<\/strong>&nbsp;This session will describe the steps in droplet-based scRNA seq data generation.<br><br>Why does this matter?<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>The analysis and troubleshooting in particular relies on understanding the library generation and cell isolation processes<\/li>\n\n\n\n<li>Also, kits! You&#8217;d be surprised how few highly experienced wet-labbers actually know what their kit is doing\u2026<\/li>\n<\/ul>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Introduce yourself to the group<\/li>\n\n\n\n<li>Describe your ideal weather<\/li>\n\n\n\n<li>Open your Breakout Room\u2019s activity using the&nbsp;<img decoding=\"async\" src=\"https:\/\/a.slack-edge.com\/production-standard-emoji-assets\/14.0\/google-medium\/1f4c1.png\" alt=\":file_folder:\"><a target=\"_blank\" href=\"https:\/\/drive.google.com\/drive\/folders\/1KoewOoaUuQjiW-vBi984uhPl326jMoP1?usp=sharing\" rel=\"noreferrer noopener\">Activity Folder<\/a>&nbsp;in the course handbook<\/li>\n\n\n\n<li>Put the labels in order, from cells to sequencing<\/li>\n\n\n\n<li>Put the pieces of the protocol in order<\/li>\n\n\n\n<li>You will be returned 15 minutes early (12:00-GMT)<\/li>\n\n\n\n<li>Message the slack&nbsp;<a target=\"_blank\" href=\"https:\/\/singlecellrna-y8w2174.slack.com\/archives\/C0851H8H92T\" rel=\"noreferrer noopener\">#course_discussion<\/a>&nbsp;channel if you finish early<\/li>\n<\/ol>\n\n\n\n<p><strong>Learning objectives:<\/strong><\/p>\n\n\n\n<p>By the end of the session you will be able to:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Describe the library generation and cell isolation process.<\/li>\n<\/ul>\n\n\n\n<p><strong>Materials:<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><mark><span style=\"color:#000000\" class=\"tadv-color\"><span style=\"background-color:#ffffff\" class=\"tadv-background-color\"><a href=\"https:\/\/docs.google.com\/presentation\/d\/12pnEfVqHN3U8DcQr1ew6E8DFdwCylZD-GHIgpoRHyI4\/edit?usp=sharing\">Presentation slides<\/a><\/span><\/span><\/mark><\/li>\n\n\n\n<li><a href=\"https:\/\/embl-ebi.cloud.panopto.eu\/Panopto\/Pages\/Viewer.aspx?id=5c733949-82b8-43c8-8569-b28700cbead5\" target=\"_blank\" rel=\"noreferrer noopener\">Recorded session<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Monday 17 February 2025 Trainer: Wendi Bacon Overview:&nbsp;This session will describe the steps in droplet-based scRNA seq data generation. Why does this matter? Learning objectives: By the end of the session you will be able to: Materials:<\/p>\n","protected":false},"author":6,"featured_media":0,"parent":12,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-273","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-handbook-2025\/wp-json\/wp\/v2\/pages\/273","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-handbook-2025\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-handbook-2025\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-handbook-2025\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-handbook-2025\/wp-json\/wp\/v2\/comments?post=273"}],"version-history":[{"count":34,"href":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-handbook-2025\/wp-json\/wp\/v2\/pages\/273\/revisions"}],"predecessor-version":[{"id":3291,"href":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-handbook-2025\/wp-json\/wp\/v2\/pages\/273\/revisions\/3291"}],"up":[{"embeddable":true,"href":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-handbook-2025\/wp-json\/wp\/v2\/pages\/12"}],"wp:attachment":[{"href":"https:\/\/www.ebi.ac.uk\/training\/materials\/single-cell-rna-seq-analysis-using-python-handbook-2025\/wp-json\/wp\/v2\/media?parent=273"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}