EMBL-EBI Training
Single cell RNA-seq analysis using Python 2025
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Single cell RNA-seq analysis using Python 2025
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Overview
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Introduction
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Course organisers
Trainer biographies
Participant biographies
Code of conduct
Pre-course challenges
Programme
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Day one
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Welcome to EMBL-EBI and introductions
Introduction to single cell technologies
Wet-lab overview
Dry-lab overview
Experimental Design and Q&A
Day two
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Designing your analysis
Getting set up: infrastructure terms
Raw reads to expression matrix
QC, pre-processing and normalisation
Feature selection, dimensionality reduction, clustering — Talk and Q&A
Batch correction and data set integration – Talk and Q&A
Day three
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Feature Selection, Dimensionality Reduction, and Data Integration — Practicals
Clustering and Cluster Marker Genes — Practicals
Differential Expression and Differential Abundance Analysis
Day four
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Spatial transcriptomics
Single Cell Expression Atlas and Single cell data submission
Group project
Day five
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Group project
Course wrap-up and survey
Posters and networking
Help sheets
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Zoom
Gather Town
Slack
Virtual machines
Further learning
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