Course content

To see details about the live Introduction to RNA-seq and functional interpretation course and the original programme, you can refer to the course page and programme.

The contents table below outlines the different sessions that were included in the course, as well as the format of the materials (presentations, videos, practicals, etc.) and each session’s trainers. Either proceed through the materials using the arrows at the end of each page or pick your topic of interest from the table below to skip straight to it.

 Presentation slides –  View or download the slides

 Recorded lectures – Watch the lecture videos 

 Practicals – View or download the slides, exercises, datasets, analysis scripts, and results

FormatSubjectTrainer
RNA-Seq introduction and fundamentals
Experimenta designVladimir Benes
Introduction to sequencingVladimir Benes
RNA-seq QC and downstream analysis
File formats and assessing quality of data (in terms of base calling)Sarah Inglesfield
Reference-based approaches to RNA-seq analysisSimon Lewis
De novo approaches to RNA-Seq (practical)Victor Flores-López and Alison Díaz-Cuevas
Annotating the genome with RNA-seq dataVictor Flores-López and Alison Díaz-Cuevas
Next steps in gene prioritisationIan Sealy
RNA-Seq data best practices and data bases
Sequencing repositoriesSarah Inglesfield
Data reproducibility: standards and ontologies –lecture and activitySilvie Fexova
Exploring gene expression dataLiora Vilmovsky
Pipeline implementation workshop
Pipiline implementation workshopGeraldine Van der Auwera