HADDOCK tutorial

Trainer: Alexandre Bonvin

Overview: This tutorial will demonstrate the use of HADDOCK for predicting the structure of a protein-protein complex from NMR chemical shift perturbation (CSP) data. Namely, you will dock two E. coli proteins involved in glucose transport: the glucose-specific enzyme IIA (E2A) and the histidine-containing phosphocarrier protein (HPr).

Learning outcomes

By the end of this session you will be able to:

• Prepare a structure for docking
• Use the HADDOCK webserver to submit docking jobs
• Visualise your docking results
• Analyse your docking results and gain biological insights from these

Materials

• Link to the tutorial