Bulk RNA-seq analysis

Trainer: Marta Perez Alcantara

Overview: This session will introduce bulk RNA-seq analysis using R and DESeq2. We will learn about quality control, dimensionality reduction, differential expression analysis, and visualisations.

Learning outcomes

By the end of this session you will be able to:

• Understand bulk RNA-seq analysis workflow
• Define important aspects of experimetal design for bulk RNA-seq analysis
• Perform quality control, dimensionality reduction and differential expression analysis

Materials:

• Lecture:

• Presentation slides

Practical Materials:

In this session, we will use DESeq2 to analyse bulk RNA-seq data. We will learn about quality control, dimensionality reduction, differential expression analysis, and visualisations.

• Tutorial: https://mperalc.gitlab.io/bulk_RNA-seq_workshop_2021/
• Links to papers can be found within the tutorials