Virtual course
Microscopy data analysis: Machine learning and the BioImage Archive
Develop programmatic skills for the analysis of bioimage data.
This course will introduce programmatic approaches used in the analysis of bioimage data via the BioImage Archive. The content will explore a variety of data types including electron microscopy, cell and tissue microscopy, and miscellaneous or multi-modal imaging data. Participants will cover contemporary biological image analysis with an emphasis on machine learning and advanced image analysis. Further instruction will be offered using applications such as ZeroCostDL4Mic, ilastik, the BioImage Model Zoo, and CellProfiler.
The organisers welcome applications from scientists who have specific research questions that look to use publicly available data held in the BioImage Archive and can demonstrate use cases for the resource.
Virtual course
Participants will learn via a mix of pre-recorded lectures, live presentations, and trainer Q&A sessions. Practical experience will be developed through group activities and trainer-led computational exercises. Live sessions will be delivered using Zoom with additional support and asynchronous communication via Slack.
Pre-recorded material may be provided before the course starts that participants will need to watch, read or work through to gain the most out of the actual training event. In the week before the course, there will be a brief induction session. Computational practicals will run on EMBL-EBI's virtual training infrastructure, meaning participants will not require access to a powerful computer or install complex software on their own machines.
Participants will need to be available between the hours of 09:30-17:30 BST each day of the course. Trainers will be available to assist, answer questions, and provide further explanations during these times.
Who is this course for?
This course is aimed at scientists working with bioimage data across the life sciences. It is suitable for those involved in creating bioimages or taking their first steps in analysis. The content would also be suitable for those wanting to learn more about the BioImage Archive and gain experience with machine learning approaches for image analysis. The programme will be of particular interest to bio-image analysts with questions relating to the use of ‘big data’ and using the wealth of publically available data curated in the BioImageArchive.
The course should be accessible to members of the bioimaging community and does not require prior experience with machine learning methods or use of the BioImage Archive is necessary, but applicants are encouraged to explore the resources below before starting their application. Applicants should be comfortable with basic programming tasks and have experience working with Python.
Prerequisite reading:
- Nature: BioImage Archive: A call for public archives for biological image data
- biorxiv: ZeroCostDL4Mic: an open platform to simplify access and use of Deep-Learning in Microscopy
- Nucleic Acids Research: The BioStudies database—one stop shop for all data supporting a life sciences study
- Nature Methods: EMPIAR: a public archive for raw electron microscopy image data
- Nature: Image Data Resource: a bioimage data integration and publication platform
- BioModelZoo
What will I learn?
Learning outcomes
After this course you should be able to:
- Interact programmatically with the BioImage Archive and other data resources
- Apply pre-built machine learning models to image data
- Train and retrain machine learning models on image data
- Utilise machine learning approaches for object detection, image segmentation and de-noising
- Generate quantitative conclusions from images
Course content
During this course you will learn about:
- Data repositories
- Analysis tools
Course funding
This course is funded in part by the Wellcome grant "The Image Data Resource: Making Biological Imaging Data FAIR", award number 212962/Z/18/Z.
Trainers
Alvis Brazma
EMBL-EBI Virginie Uhlmann
EMBL-EBI Craig Russell
EMBL-EBI Ricardo Henriques
UCL Romain Laine
UCL Soham Mandal
EMBL-EBI Ugis Sarkans
EMBL-EBI Awais Athar
EMBL-EBI Matthew Hartley
EMBL-EBI Ardan Patwardhan
EMBL-EBI Osman Salih
EMBL-EBI Andrii Iudin
EMBL-EBI Simone Weyand
EMBL-EBI Jason Swedlow
University of Dundee Jean Marie Burel
University of Dundee Anna Kreshuk
EMBL Beth Cimini
Broad Institute Wei Ouyang
SciLifeLAb Esi Gomez de Mariscal
UC3M Dominik Kutra
EMBL Petr Walczysko
University of Dundee Frances Wong
University of Dundee Nasim Jamali
Broad Institute David Stirling
Broad Institue Barbara Diaz-Rohrer
Broad Institute Martin Weigert
EPFL Guillaume Jacquemet
University of Turku Gerard Kleywegt
EMBL-EBI
Programme
All times are given in British Summer Time (BST).
Day 1 - Monday 12 July
09:30-09:45
Arrival and networking hangouts
09:45-10:00
Welcome and overview of virtual training
Pati Carvajal Lopez & Alex Holinski
10:00-10:30
Introduction to image analysis
Alvis Brazma & Virginie Uhlmann
10:30-10:45
Break
10:45-11:30
ZeroCostDL4Mic - lecture and questions
Ricardo Henriques, Romain Laine, Guillaume Jacquemet
11:30-12:30
Segmentation with StarDist and SplineDist - lecture and questions
Soham Mandal, Virginie Uhlmann, Martin Weigert
12:30-13:00
Flash talks & discussion
13:00-13:25
Break
13:25-15:00
Practical session
Romain Laine, Craig Russell, Virginie Uhlmann, Soham Mandal
15:00-15:15
Break
15:15-16:30
Practical session
Romain Laine, Craig Russell, Virginie Uhlmann, Soham Mandal
16:30-17:00
Discussion and networking
17:00
End of day
Day 2 - Tuesday 13 July
09:30-10:00
Arrival and networking hangouts
10:00-11:00
BioStudies - lecture and questions
Ugis Sarkans & Awais Athar
11:00-11:15
Break
11:15-12:30
Variety - lecture and questions
Matthew Hartley & Craig Russell
12:30-13:00
Flash talks & discussion
13:00-13:30
Break
13:30-15:30
Practical session
Craig Russell, Romain Laine & Guillaume Jacquemet
15:30-15:45
Break
15:45-16:30
Practical session
Craig Russell, Romain Laine & Guillaume Jacquemet
16:30-17:00
Discussion and networking
17:00
End of day
Day 3 - Wednesday 14 July
09:30-10:00
Arrival and networking hangouts
10:00-11:00
EMPIAR: past, present and future - lecture and questions
Gerard Kleywegt
11:00-11:15
Break
11:15-12:30
Depositing and accessing data at EMPIAR - lecture and questions
Osman Salih
12:30-13:00
Flash talks & discussion
13:00-13:30
Break
13:30-15:00
Depositing and accessing data at EMPIAR - practical
Andrii Iudin, Osman Salih & Simone Weyand
15:00-15:15
Break
15:15-16:30
Depositing and accessing data at EMPIAR - practical
Andrii Iudin, Osman Salih & Simone Weyand
16:30-17:00
Discussion and networking
All
17:00
End of day
Day 4 - Thursday 15 July
09:30-10:00
Arrival and networking hangouts
10:00-11:00
Image data resource - lecture + Q&A
Jason Swedlow
11:00-11:15
Break
11:15-12:30
Image data resource - lecture and questions
Frances Wong
12:30-13:00
Flash talks & discussion
13:00-13:30
Break
13:30-15:30
Image data resource - practical
Jean Marie Burel, Petr Walczysko, Frances Wong
15:30-15:45
Break
15:45-16:30
Discussion and networking
All
17:00
End of day
Day 5 - Friday 16 July
09:30-10:00
Arrival and networking hangouts
10:00-11:00
BioImage Model Zoo - lecture and questions
Anna Kreshuk
11:00-11:15
Break
11:15-13:15
BioImage Model Zoo - practical
ilastik team, Wei Ouyang, Esti Gomez de Mariscal, Dominik Kutra
13:15-13:45
Break
13:45-14:45
CellProfiler - lecture and questions
Beth Cimini
14:45-15:00
Break
15:00-16:30
CellProfiler - practical
Nasim Jamali, David Stirling, Barbara Diaz-Rohrer
16:30-17:00
Discussion and course wrap-up
All
17:00
End of day
Please read our application advice before starting your application. In order to be considered for a place on this course, you must do the following:
- Complete the online application form
- Provide a Microsoft Word (.docx) document containing three short paragraphs with a biography, work history and description of your current research interests; each paragraph should be no more than 100 words.
- Provide a letter of support from a supervisor or a senior co-worker explaining why you should be selected for this course.
Please complete the online application form by 23:59 (BST) Friday 14 May.
Incomplete applications will not be considered.
All applicants will be informed of the status of their application (successful, waiting list, rejected) no later than Tuesday 1 May.
Participant Flash Talks
All participants will be asked to give a short presentation about their research work as part of the course. These provide an opportunity to share their research with the other participants and provide a forum for discussion. Further details will be provided following registration.
EMBL-EBI
EMBL-EBI
EMBL-EBI
UCL
UCL
EMBL-EBI
EMBL-EBI
EMBL-EBI
EMBL-EBI
EMBL-EBI
EMBL-EBI
EMBL-EBI
EMBL-EBI
University of Dundee
University of Dundee
EMBL
Broad Institute
SciLifeLAb
UC3M
EMBL
University of Dundee
University of Dundee
Broad Institute
Broad Institue
Broad Institute
EPFL
University of Turku
EMBL-EBI
Programme
All times are given in British Summer Time (BST).
Day 1 - Monday 12 July |
||
09:30-09:45 | Arrival and networking hangouts | |
09:45-10:00 | Welcome and overview of virtual training | Pati Carvajal Lopez & Alex Holinski |
10:00-10:30 | Introduction to image analysis | Alvis Brazma & Virginie Uhlmann |
10:30-10:45 | Break | |
10:45-11:30 | ZeroCostDL4Mic - lecture and questions | Ricardo Henriques, Romain Laine, Guillaume Jacquemet |
11:30-12:30 | Segmentation with StarDist and SplineDist - lecture and questions | Soham Mandal, Virginie Uhlmann, Martin Weigert |
12:30-13:00 | Flash talks & discussion | |
13:00-13:25 | Break | |
13:25-15:00 | Practical session | Romain Laine, Craig Russell, Virginie Uhlmann, Soham Mandal |
15:00-15:15 | Break | |
15:15-16:30 | Practical session | Romain Laine, Craig Russell, Virginie Uhlmann, Soham Mandal |
16:30-17:00 | Discussion and networking | |
17:00 | End of day | |
Day 2 - Tuesday 13 July |
||
09:30-10:00 | Arrival and networking hangouts | |
10:00-11:00 | BioStudies - lecture and questions | Ugis Sarkans & Awais Athar |
11:00-11:15 | Break | |
11:15-12:30 | Variety - lecture and questions | Matthew Hartley & Craig Russell |
12:30-13:00 | Flash talks & discussion | |
13:00-13:30 | Break | |
13:30-15:30 | Practical session | Craig Russell, Romain Laine & Guillaume Jacquemet |
15:30-15:45 | Break | |
15:45-16:30 | Practical session | Craig Russell, Romain Laine & Guillaume Jacquemet |
16:30-17:00 | Discussion and networking | |
17:00 | End of day | |
Day 3 - Wednesday 14 July |
||
09:30-10:00 | Arrival and networking hangouts | |
10:00-11:00 | EMPIAR: past, present and future - lecture and questions | Gerard Kleywegt |
11:00-11:15 | Break | |
11:15-12:30 | Depositing and accessing data at EMPIAR - lecture and questions | Osman Salih |
12:30-13:00 | Flash talks & discussion | |
13:00-13:30 | Break | |
13:30-15:00 | Depositing and accessing data at EMPIAR - practical | Andrii Iudin, Osman Salih & Simone Weyand |
15:00-15:15 | Break | |
15:15-16:30 | Depositing and accessing data at EMPIAR - practical | Andrii Iudin, Osman Salih & Simone Weyand |
16:30-17:00 | Discussion and networking | All |
17:00 | End of day | |
Day 4 - Thursday 15 July |
||
09:30-10:00 | Arrival and networking hangouts | |
10:00-11:00 | Image data resource - lecture + Q&A | Jason Swedlow |
11:00-11:15 | Break | |
11:15-12:30 | Image data resource - lecture and questions | Frances Wong |
12:30-13:00 | Flash talks & discussion | |
13:00-13:30 | Break | |
13:30-15:30 | Image data resource - practical | Jean Marie Burel, Petr Walczysko, Frances Wong |
15:30-15:45 | Break | |
15:45-16:30 | Discussion and networking | All |
17:00 | End of day | |
Day 5 - Friday 16 July |
||
09:30-10:00 | Arrival and networking hangouts | |
10:00-11:00 | BioImage Model Zoo - lecture and questions | Anna Kreshuk |
11:00-11:15 | Break | |
11:15-13:15 | BioImage Model Zoo - practical | ilastik team, Wei Ouyang, Esti Gomez de Mariscal, Dominik Kutra |
13:15-13:45 | Break | |
13:45-14:45 | CellProfiler - lecture and questions | Beth Cimini |
14:45-15:00 | Break | |
15:00-16:30 | CellProfiler - practical | Nasim Jamali, David Stirling, Barbara Diaz-Rohrer |
16:30-17:00 | Discussion and course wrap-up | All |
17:00 | End of day |
Please read our application advice before starting your application. In order to be considered for a place on this course, you must do the following:
- Complete the online application form
- Provide a Microsoft Word (.docx) document containing three short paragraphs with a biography, work history and description of your current research interests; each paragraph should be no more than 100 words.
- Provide a letter of support from a supervisor or a senior co-worker explaining why you should be selected for this course.
Please complete the online application form by 23:59 (BST) Friday 14 May.
Incomplete applications will not be considered.
All applicants will be informed of the status of their application (successful, waiting list, rejected) no later than Tuesday 1 May.
Participant Flash Talks
All participants will be asked to give a short presentation about their research work as part of the course. These provide an opportunity to share their research with the other participants and provide a forum for discussion. Further details will be provided following registration.