Virtual course
Microscopy data analysis: machine learning and the BioImage Archive
This course will introduce programmatic approaches used in the analysis of bioimage data via the BioImage Archive. The content will explore a variety of data types including electron microscopy, cell and tissue microscopy, and miscellaneous or multi-modal imaging data. Participants will cover contemporary biological image analysis with an emphasis on machine learning and advanced image analysis. Further instruction will be offered using applications such as ZeroCostDL4Mic, ilastik, ImJoy, the BioImage Model Zoo, and CellProfiler.
Virtual course
Participants will learn via a mix of pre-recorded lectures, live presentations, and trainer Q&A sessions. Practical experience will be developed through group activities and trainer-led computational exercises. Live sessions will be delivered using Zoom with additional support and asynchronous communication via Slack.
Pre-recorded material may be provided before the course starts that participants will need to watch, read or work through to gain the most out of the actual training event. In the week before the course, there will be a brief induction session. Computational practicals will run on EMBL-EBI's virtual training infrastructure, meaning participants will not require access to a powerful computer or install complex software on their own machines.
Participants will need to be available between the hours of 09:00 - 17:30 BST each day of the course. Trainers will be available to assist, answer questions, and provide further explanations during these times.
Who is this course for?
This course is aimed at scientists working with biomage data across the life sciences. It is suitable for those involved in creating bioimages or taking their first steps in analysis. The content would also be suitable for those wanting to learn more about the BioImage Archive and gain experience with machine learning approaches for image analysis. The programme will be of particular interest to bioimage analysts with questions relating to the use of ‘big data’ and using the wealth of publically available data curated in the BioImage Archive.
The course should be accessible to members of the bioimaging community and does not require prior experience with machine learning methods or use of the BioImage Archive. Applicants are encouraged to explore the resources below before starting their application. Applicants should be comfortable with basic programming tasks and have experience working with Python.
Prerequisite reading:
- BioImage Archive: A call for public archives for biological image data
- ZeroCostDL4Mic: an open platform to simplify access and use of Deep-Learning in Microscopy
- The BioStudies database - one stop shop for all data supporting a life sciences study
- EMPIAR: a public archive for raw electron microscopy image data
- Image Data Resource: a bioimage data integration and publication platform
- BioImage Model Zoo
What will I learn?
Learning outcomes
After this course you should be able to:
- Interact programmatically with the BioImage Archive and other data resources
- Apply pre-built machine learning models to image data
- Train and retrain machine learning models on image data
- Utilise machine learning approaches for object detection, image segmentation and de-noising
- Generate quantitative conclusions from images
Course content
During this course you will learn about:
Data repositories
Analysis tools
Trainers
Awais Athar
EMBL-EBI Alvis Brazma
EMBL-EBI Jean Marie Burel
University of Dundee Beth Cimini
Broad Institute Mario Costa Cruz
Broad Institute Barbara Diaz-Rohrer
Broad Institute Esti Gomez de Mariscal
UC3M Matthew Hartley
EMBL-EBI Andrii Iudin
EMBL-EBI Guillame Jacquemet
UTU Gerard Kleywegt
EMBL-EBI Anna Kreshuk
EMBL Dominik Kutra
EMBL Soham Mandal
EMBL-EBI Wei Ouyang
SciLifeLab Craig Russell
EMBL-EBI Osman Salih
EMBL-EBI Ugis Sarkans
EMBL-EBI Rebecca Senft
Broad Institute Jason Swedlow
University of Dundee Callum Tromans-Coia
Broad Institute Virginie Uhlmann
EMBL-EBI Petr Walczysko
University of Dundee Martin Weigert
EPFL Simone Weyand
EMBL-EBI Frances Wong
University of Dundee Timo Zimmermann
EMBL Paul Korir
EMBL-EBI Sriram Sundar Somasundharam
EMBL-EBI
Programme
Time
Topic
Trainer
Day 1 – 16 May 2022
09:30 - 09:45
Arrival and networking hangouts
09:45 - 10:00
Welcome and overview of virtual training
Patricia Carvajal-López
10:00 - 10:30
Introduction to image analysis
Alvis Brazma, Matthew Hartley and Virginie Uhlmann
10:30 - 10:45
Break
10:45 - 11:30
Deep learning introduction
Guillame Jacquemet and Soham Mandal
11:30 - 12:30
Segmentation with StarDist and SplineDist - lecture and questions
Soham Mandal, Virginie Uhlmann and Martin Weigert
12:30 - 13:30
Flash talks and discussion
13:30 - 14:30
Break
14:30 - 15:30
ZeroCost - Practical
Guillame Jacquemet, Soham Mandal, Esti Gómez de Mariscal
15:30 - 15:45
Break
15:45 - 16:30
Terminal for scientific computing (git, bash and more) - Practical
Craig Russell
16:30 - 17:00
Discussion and networking
17:00
End of day
Day 2 – 17 May 2022
09:30 - 10:00
Arrival and networking hangouts
10:00 -1 0:45
BioStudies - lecture and questions
Ugis Sarkans and Awais Athar
10:45 - 11:00
Break
11:00 - 12:30
BioImage Archive
Matthew Hartley and Craig Russell
12:30 - 13:30
Flash talks and discussion
13:30 - 14:30
Break
14:30 - 15:30
Scientific pipelines - practical session
Craig Russell and Jean-Marie Burel
15:30 - 15:45
Break
15:45 - 16:30
ImJoy - practical session
Wei Ouyang
16:30 - 17:00
Discussion and networking
17:00
End of day
Day 3 – 18 May 2022
09:30 - 10:00
Arrival and networking hangouts
10:00 - 11:00
EMPIAR: past, present and future - lecture and questions
Gerard Kleywegt
11:00 - 11:15
Break
11:15 - 12:30
Depositing and accessing data at EMPIAR - Practical
Osman Salih, Andrii Iudin, Simone Weyand, Paul Korir, Sriram Somasundharam
12:30 - 13:30
Flash talks & discussion
13:30 - 14:30
Break
14:30 - 15:30
Distributed/Cloud computing - practical
Jean-Marie Burel, Petr Walczysko
15:30 - 15:45
Break
15:45 - 16:30
Distributed/Cloud computing - practical continuation
Jean-Marie Burel, Petr Walczysko
16:30 - 17:00
Discussion and networking
17:00
End of day
Day 4 – 19 May 2022
09:30 - 10:00
Arrival and networking hangouts
10:00 - 11:00
Image data resource - lecture and Q&A
Jason Swedlow
11:00 - 11:15
Break
11:15 - 12:30
Image data resource - lecture and questions
Frances Wong
12:30 - 13:30
Flash talks & discussion
13:30 - 14:30
Break
14:30 - 15:30
Image data resource - practical
Jean Marie Burel, Petr Walczysko and Frances Wong
15:30 - 15:45
Break
15:45 - 16:30
The EMBL Imaging Centre
Timo Job Kristian Zimmermann
16:30 - 17:00
End of day
Day 5 – 20 May 2022
09:30 - 10:00
Arrival and networking hangouts
10:00 - 11:00
BioImage Model Zoo - lecture and questions
Anna Kreshuk
11:00 - 11:15
Break
11:15 - 13:15
BioImage Model Zoo - practical
ilastik team, Esti Gomez de Mariscal and Dominik Kutra
13:15 - 13:45
Break
13:45 - 14:45
CellProfiler - lecture and questions
Beth Cimini
14:45 - 15:00
Break
15:00 - 16:30
CellProfiler - practical
Barbara Diaz-Rohrer, Rebecca Senft, Mario Costa Cruz, Callum Tromans-Coia
16:30 - 17:00
Discussion and course wrap-up
17:00
End of day
Please read our application advice before starting your application. In order to be considered for a place on this course, you must do the following:
- Complete the online application form
- Provide a PDF or Microsoft Word (.docx) document containing three short paragraphs with a biography, work history and description of your current research interests; each paragraph should be no more than 100 words.
- Provide a letter of support from a supervisor or a senior co-worker explaining why you should be selected for this course.
Please complete the online application form by 11 February 2022.
Incomplete applications will not be considered.
All applicants will be informed of the status of their application (successful, waiting list, rejected) no later on 24 February 2022.
Participant flash talks
All participants will be asked to give a short presentation about their research work as part of the course. These provide an opportunity to share their research with the other participants and provide a forum for discussion. Further details will be provided following registration.
Course materials
The course materials from the 2021 edition of the course are now live and available for you to use. They provide a mixture of pre-recorded lectures, presentations and practicals from the course, and will give you a snapshot of what to expect in the 2022 edition.
EMBL-EBI
EMBL-EBI
University of Dundee
Broad Institute
Broad Institute
Broad Institute
UC3M
EMBL-EBI
EMBL-EBI
UTU
EMBL-EBI
EMBL
EMBL
EMBL-EBI
SciLifeLab
EMBL-EBI
EMBL-EBI
EMBL-EBI
Broad Institute
University of Dundee
Broad Institute
EMBL-EBI
University of Dundee
EPFL
EMBL-EBI
University of Dundee
EMBL
EMBL-EBI
EMBL-EBI
Programme
| Time | Topic | Trainer | |
| Day 1 – 16 May 2022 | |||
| 09:30 - 09:45 | Arrival and networking hangouts | ||
| 09:45 - 10:00 | Welcome and overview of virtual training | Patricia Carvajal-López | |
| 10:00 - 10:30 | Introduction to image analysis | Alvis Brazma, Matthew Hartley and Virginie Uhlmann | |
| 10:30 - 10:45 | Break | ||
| 10:45 - 11:30 | Deep learning introduction | Guillame Jacquemet and Soham Mandal | |
| 11:30 - 12:30 | Segmentation with StarDist and SplineDist - lecture and questions | Soham Mandal, Virginie Uhlmann and Martin Weigert | |
| 12:30 - 13:30 | Flash talks and discussion | ||
| 13:30 - 14:30 | Break | ||
| 14:30 - 15:30 | ZeroCost - Practical | Guillame Jacquemet, Soham Mandal, Esti Gómez de Mariscal | |
| 15:30 - 15:45 | Break | ||
| 15:45 - 16:30 | Terminal for scientific computing (git, bash and more) - Practical | Craig Russell | |
| 16:30 - 17:00 | Discussion and networking | ||
| 17:00 | End of day | ||
| Day 2 – 17 May 2022 | |||
| 09:30 - 10:00 | Arrival and networking hangouts | ||
| 10:00 -1 0:45 | BioStudies - lecture and questions | Ugis Sarkans and Awais Athar | |
| 10:45 - 11:00 | Break | ||
| 11:00 - 12:30 | BioImage Archive | Matthew Hartley and Craig Russell | |
| 12:30 - 13:30 | Flash talks and discussion | ||
| 13:30 - 14:30 | Break | ||
| 14:30 - 15:30 | Scientific pipelines - practical session | Craig Russell and Jean-Marie Burel | |
| 15:30 - 15:45 | Break | ||
| 15:45 - 16:30 | ImJoy - practical session | Wei Ouyang | |
| 16:30 - 17:00 | Discussion and networking | ||
| 17:00 | End of day | ||
| Day 3 – 18 May 2022 | |||
| 09:30 - 10:00 | Arrival and networking hangouts | ||
| 10:00 - 11:00 | EMPIAR: past, present and future - lecture and questions | Gerard Kleywegt | |
| 11:00 - 11:15 | Break | ||
| 11:15 - 12:30 | Depositing and accessing data at EMPIAR - Practical | Osman Salih, Andrii Iudin, Simone Weyand, Paul Korir, Sriram Somasundharam | |
| 12:30 - 13:30 | Flash talks & discussion | ||
| 13:30 - 14:30 | Break | ||
| 14:30 - 15:30 | Distributed/Cloud computing - practical | Jean-Marie Burel, Petr Walczysko | |
| 15:30 - 15:45 | Break | ||
| 15:45 - 16:30 |
|
Jean-Marie Burel, Petr Walczysko | |
| 16:30 - 17:00 | Discussion and networking | ||
| 17:00 | End of day | ||
| Day 4 – 19 May 2022 | |||
| 09:30 - 10:00 | Arrival and networking hangouts | ||
| 10:00 - 11:00 | Image data resource - lecture and Q&A | Jason Swedlow | |
| 11:00 - 11:15 | Break | ||
| 11:15 - 12:30 | Image data resource - lecture and questions | Frances Wong | |
| 12:30 - 13:30 | Flash talks & discussion | ||
| 13:30 - 14:30 | Break | ||
| 14:30 - 15:30 | Image data resource - practical | Jean Marie Burel, Petr Walczysko and Frances Wong | |
| 15:30 - 15:45 | Break | ||
| 15:45 - 16:30 | The EMBL Imaging Centre | Timo Job Kristian Zimmermann | |
| 16:30 - 17:00 | End of day | ||
| Day 5 – 20 May 2022 | |||
| 09:30 - 10:00 | Arrival and networking hangouts | ||
| 10:00 - 11:00 | BioImage Model Zoo - lecture and questions | Anna Kreshuk | |
| 11:00 - 11:15 | Break | ||
| 11:15 - 13:15 | BioImage Model Zoo - practical | ilastik team, Esti Gomez de Mariscal and Dominik Kutra | |
| 13:15 - 13:45 | Break | ||
| 13:45 - 14:45 | CellProfiler - lecture and questions | Beth Cimini | |
| 14:45 - 15:00 | Break | ||
| 15:00 - 16:30 | CellProfiler - practical | Barbara Diaz-Rohrer, Rebecca Senft, Mario Costa Cruz, Callum Tromans-Coia | |
| 16:30 - 17:00 | Discussion and course wrap-up | ||
| 17:00 | End of day | ||
Please read our application advice before starting your application. In order to be considered for a place on this course, you must do the following:
- Complete the online application form
- Provide a PDF or Microsoft Word (.docx) document containing three short paragraphs with a biography, work history and description of your current research interests; each paragraph should be no more than 100 words.
- Provide a letter of support from a supervisor or a senior co-worker explaining why you should be selected for this course.
Please complete the online application form by 11 February 2022.
Incomplete applications will not be considered.
All applicants will be informed of the status of their application (successful, waiting list, rejected) no later on 24 February 2022.
Participant flash talks
All participants will be asked to give a short presentation about their research work as part of the course. These provide an opportunity to share their research with the other participants and provide a forum for discussion. Further details will be provided following registration.
Course materials
The course materials from the 2021 edition of the course are now live and available for you to use. They provide a mixture of pre-recorded lectures, presentations and practicals from the course, and will give you a snapshot of what to expect in the 2022 edition.