Course at EMBL-EBI
Managing single cell transcriptomics data
This course aims to provide researchers generating and working with single-cell RNA sequencing or image-based transcriptomics data with the tools and knowledge for generating high-quality metadata alongside their data and analyses. Participants will learn what it means to make their data FAIR (Findable, Accessible, Interoperable, and Reusable) and what tools and standards are available to help them with good data management and FAIRification. Finally, participants will learn about the Human Cell Atlas project and how to ensure their data reaches the wider research community.
Who is this course for?
This course is aimed at researchers who are generating, planning on generating, or working with single cell RNA sequencing or image-based transcriptomics data. This course will not cover any aspects of data analysis, therefore no prior computational knowledge is required.
What will I learn?
Learning outcomes
After this course, you should be able to:
- Describe single cell RNA sequencing and image-based transcriptomic technologies and apply appropriate experimental approaches
- Define best practice for managing cellular resolution data
- Know which standards for data and metadata need to be applied to data being generated
- Choose appropriate ontologies for annotation of data
- Explore the HCA and prepare quality data to be submitted to the Atlas
Course content
During this course you will learn about:
- General principles of data management, data FAIRification and best practice for generating and working with single cell RNA sequencing and image-based transcriptomics data
- Experimental design for single cell experiments
- HCA data and metadata standards
Trainers
Danielle Welter
EMBL-EBI, UK Mallory Freeberg
EMBL-EBI, UK Zinaida Perova
EMBL-EBI, UK Sarah Morgan
EMBL-EBI, UK Wendi Bacon
EMBL-EBI, UK Brett McClintock
EMBL-EBI, UK Alfonso Munoz-Pomer Fuentes
EMBL-EBI, UK
Programme
Day 1 – Wednesday 3rd July 2019
12:00 – 13:00
Arrival, registration and lunch
13:00 – 13:30
Welcome to EMBL-EBI and introductions
Wendi Bacon
13:30 – 14:00
Participant introductions
Wendi Bacon
14:00 – 15:00
What is ‘good data management’ and why do we need it?
Wendi Bacon
15:00 – 15:30
Break
15:30 – 16:00
Data management: Tales from the Lab Bench
Wendi Bacon
16:00 – 17:30
FAIRifying your data for publication using ontologies and standards
Dani Welter & Mallory Freeberg
17:30 – 18:00
Group discussions
Wendi Bacon & Mallory Freeberg
18:00
End of day
18:15
Check into accommodation
19:00
Dinner at Hinxton Hall
Day 2 – Thursday 4th July 2019
9:00 – 9:30
What's a server? Computational terms & other questionable questions
Wendi Bacon, Alfonso Fuentes, Brett McClintock
9:30 – 11:00
Single cell RNA sequencing technology overview
Wendi Bacon
11:00 – 11:30
Break
11:30 – 13:00
Experimental design considerations
Lia Chappell & Jenni Westoby
13:00 – 14:00
Lunch
14:00 – 15:00
Spatially resolved transcriptomics technology overview
Zina Perova
15:00 – 15:30
Break
15:30 – 17:00
Experimental design considerations
Zina Perova
17:00 – 17:30
Group discussions
Wendi Bacon & Zina Perova
17:30
End of day
18:30
Dinner at Hinxton Hall
Day 3 – Friday 5th July 2019
9:00 – 9:30
HCA overview
Mallory Freeberg
9:30 – 10:30
Data and metadata standards for HCA
Dani Welter
10:30 – 11:00
Coffee
11:00 – 12:00
Submission to HCA
Mallory Freeberg
12:00 – 12:30
Discussion
Mallory Freeberg
12:30 – 13:00
Wrap-up and feedback
Mallory Freeberg & Sarah Morgan
13:00 – 13:45
Lunch
13:45
Bus to Cambridge Train Station
Attendance at this workshop is allocated on a first come, first served basis - your place is guaranteed as soon as you register (30 places available; after this you will be registered on a waiting list).
Please note that registration closes four weeks prior to the course, so please register as soon as you can. Once you have registered and we have received payment we can provide a letter of support should you require a visa to travel to the UK. Applying for a visa can take several weeks, and it might not be possible to be granted a visa if you register just before the closing date. If you are unable to attend, then please notify us as quickly as possible so that we can offer your place to someone else.
EMBL-EBI, UK
EMBL-EBI, UK
EMBL-EBI, UK
EMBL-EBI, UK
EMBL-EBI, UK
EMBL-EBI, UK
EMBL-EBI, UK
Programme
Day 1 – Wednesday 3rd July 2019 |
||
|---|---|---|
| 12:00 – 13:00 | Arrival, registration and lunch | |
| 13:00 – 13:30 | Welcome to EMBL-EBI and introductions | Wendi Bacon |
| 13:30 – 14:00 | Participant introductions | Wendi Bacon |
| 14:00 – 15:00 | What is ‘good data management’ and why do we need it? | Wendi Bacon |
| 15:00 – 15:30 | Break | |
| 15:30 – 16:00 | Data management: Tales from the Lab Bench | Wendi Bacon |
| 16:00 – 17:30 | FAIRifying your data for publication using ontologies and standards | Dani Welter & Mallory Freeberg |
| 17:30 – 18:00 | Group discussions | Wendi Bacon & Mallory Freeberg |
| 18:00 | End of day | |
| 18:15 | Check into accommodation | |
| 19:00 | Dinner at Hinxton Hall |
Day 2 – Thursday 4th July 2019 |
||
|---|---|---|
| 9:00 – 9:30 | What's a server? Computational terms & other questionable questions | Wendi Bacon, Alfonso Fuentes, Brett McClintock |
| 9:30 – 11:00 | Single cell RNA sequencing technology overview | Wendi Bacon |
| 11:00 – 11:30 | Break | |
| 11:30 – 13:00 | Experimental design considerations | Lia Chappell & Jenni Westoby |
| 13:00 – 14:00 | Lunch | |
| 14:00 – 15:00 | Spatially resolved transcriptomics technology overview | Zina Perova |
| 15:00 – 15:30 | Break | |
| 15:30 – 17:00 | Experimental design considerations | Zina Perova |
| 17:00 – 17:30 | Group discussions | Wendi Bacon & Zina Perova |
| 17:30 | End of day | |
| 18:30 | Dinner at Hinxton Hall |
Day 3 – Friday 5th July 2019 |
||
|---|---|---|
| 9:00 – 9:30 | HCA overview | Mallory Freeberg |
| 9:30 – 10:30 | Data and metadata standards for HCA | Dani Welter |
| 10:30 – 11:00 | Coffee | |
| 11:00 – 12:00 | Submission to HCA | Mallory Freeberg |
| 12:00 – 12:30 | Discussion | Mallory Freeberg |
| 12:30 – 13:00 | Wrap-up and feedback | Mallory Freeberg & Sarah Morgan |
| 13:00 – 13:45 | Lunch | |
| 13:45 | Bus to Cambridge Train Station |
Attendance at this workshop is allocated on a first come, first served basis - your place is guaranteed as soon as you register (30 places available; after this you will be registered on a waiting list).
Please note that registration closes four weeks prior to the course, so please register as soon as you can. Once you have registered and we have received payment we can provide a letter of support should you require a visa to travel to the UK. Applying for a visa can take several weeks, and it might not be possible to be granted a visa if you register just before the closing date. If you are unable to attend, then please notify us as quickly as possible so that we can offer your place to someone else.