Course outside EMBL-EBI
Exploring Biodiversity through Bioinformatics
Many Latin American countries are hotspots for biodiversity, most of which have been characterized only to a very limited extent. This course aims to provide knowledge on available approaches, methods and tools to perform studies on biodiversity employing modern high-throughput sequencing approaches. After the course, participants will have learnt how to select the appropriate sequencing approach and experimental setup, how to access, control quality and clean data, and to analyze and interpret results using available bioinformatics tools.
Who is this course for?
This course is aimed at wet-lab researchers at any career stage within Latin America who are working with and/or generating their own plant genomic and/or microbial genomic/transcriptomic datasets.
Basic computational or previous bioinformatics experience is required for this workshop, and an undergraduate level knowledge of biology would be an advantage.
A number of travel fellowships are available for this course - early-stage researchers and researchers from underrepresented groups are especially encouraged to apply for CABANA travel fellowships. You can apply for travel fellowships via the course application form.
What will I learn?
Learning outcomes
After this course you should be able to:
- Select an appropriate sequencing approach and study design to address your specific biodiversity questions
- Access a range of data types from leading public repositories
- Process sequence data from various approaches for biodiversity analysis using Galaxy pipelines
- Interpret results of DNA based biodiversity analysis
Trainers
Jan Kreuze
International Potato Center (CIP), Peru Noelle Anglin
International Potato Center (CIP), Peru Guilherme Oliveira
Instituto Tecnológico Vale - ITV, Brazil Marco Cristancho
Universidad de Los Andes, Colombia Hannele Lindqvist-Kreuze
International Potato Center (CIP), Peru
Programme
Day 1 – Tuesday 9 April
08:30-09:00
Arrival, registration
09:00-10:00
Welcome and introductions
Jan Kreuze / Noelle Anglin
10:00-11:00
An overview of different sequencing approaches to analyze biodiversity
Santelmo Vasconcelos
11:00-11:30
Coffee Break
11:30-13:00
DNA barcodes - experimental design
Santelmo Vasconcelos
13:00-14:00
Lunch
CIP Cafeteria
14:00-15:30
DNA barcodes - sampling methodology
Santelmo Vasconcelos
15:30-16:00
Coffee Break
16:00-17:30
Data cleaning
Santelmo Vasconcelos
17:30
End of day 1
Day 2 – Wednesday 10 April
08:30-09:30
What is a barcode and how to select markers
Santelmo Vasconcelos
09:30-10:30
Choosing and using markers for DNA barcodes
Santelmo Vasconcelos
10:30-11:00
Coffee Break
11:00-11:30
Choosing and using markers for DNA barcodes
Santelmo Vasconcelos
11:30-13:00
Developing new DNA barcodes
Santelmo Vasconcelos
13:00-14:00
Lunch
CIP Cafeteria
14:00-15:00
Analyzing DNA barcodes
Santelmo Vasconcelos
15:00-16:00
Exercise: obtaining a dataset, and running the analyses
Santelmo Vasconcelos
16:00-16:30
Coffee Break
16:30-17:30
Exercise: obtaining a dataset, and running the analyses
Santelmo Vasconcelos
17:30
End of day 2
Day 3 – Thursday 11 April
08:30-09:30
Phylogeny and species definition by DNA barcodes
Santelmo Vasconcelos
09:30-10:30
Exercise: phylogeny and barcode gap
Santelmo Vasconcelos
10:30-11:00
Coffee Break
11:00-11:30
Exercise: phylogeny and barcode gap
Santelmo Vasconcelos
11:30-12:00
Final Questions
Santelmo Vasconcelos
12:00-13:00
Introduction to Metagenomics data, databases and molecular data formats
Marco Cristancho
13:00-14:00
Lunch
CIP Cafeteria
14:00-15:00
Software for Metagenomics analysis
Marco Cristancho
15:00-16:00
Introduction to Galaxy
Marco Cristancho
16:00-16:30
Coffee Break
16:30-17:30
Practical: Analysis of Metagenomics Data with Galaxy
Marco Cristancho
17:30
End of day 3
18:00-20:00
Course dinner
Day 4 – Friday 12 April
08:30-09:30
Practical: Analysis of Metagenomics Data with Galaxy
Marco Cristancho
09:30-10:00
Q&A on Metagegenomics analysis
Marco Cristancho
10:00-10:30
Coffee Break
10:30-11:30
Introduction to genome wide association studies (GWAS)
Hannele Lindqvist-Kreuze
11:30-13:00
Practical: GWAS in polyploid potato using RStudio and GWASPoly
Hannele Lindqvist-Kreuze
13:00-14:00
Lunch
CIP Cafeteria
14:00-14:30
Q&A on Molecular markers
Hannele Lindqvist-Kreuze
14:30-15:00
Introduction to viral metagenomics
Jan Kreuze
15:00-16:00
Practical: viral metagenomics using VirusDetect
Jorge Villacorta
16:00-16:15
Coffee Break
16:15-17:00
Practical: viral metagenomics using VirusDetect
Jorge Villacorta
17:00-17:30
Course wrap-up and feedback
Jan Kreuze / Trainers
In order to be considered for a place on this course applicants must complete the online application form.
Incomplete applications will NOT be considered.
If you have any queries about the registration process please email Piv Gopalasingam.
The registration fee includes:
- Tea/coffee and lunches will be provided for the duration of the course
- Course dinner on Thursday 11th April
- Use of the course training room and computers
- Course materials provided
- Course certificate
International Potato Center (CIP), Peru
International Potato Center (CIP), Peru
Instituto Tecnológico Vale - ITV, Brazil
Universidad de Los Andes, Colombia
International Potato Center (CIP), Peru
Programme
Day 1 – Tuesday 9 April |
||
---|---|---|
08:30-09:00 | Arrival, registration | |
09:00-10:00 | Welcome and introductions | Jan Kreuze / Noelle Anglin |
10:00-11:00 | An overview of different sequencing approaches to analyze biodiversity | Santelmo Vasconcelos |
11:00-11:30 | Coffee Break | |
11:30-13:00 | DNA barcodes - experimental design | Santelmo Vasconcelos |
13:00-14:00 | Lunch | CIP Cafeteria |
14:00-15:30 | DNA barcodes - sampling methodology | Santelmo Vasconcelos |
15:30-16:00 | Coffee Break | |
16:00-17:30 | Data cleaning | Santelmo Vasconcelos |
17:30 | End of day 1 |
Day 2 – Wednesday 10 April |
||
---|---|---|
08:30-09:30 | What is a barcode and how to select markers | Santelmo Vasconcelos |
09:30-10:30 | Choosing and using markers for DNA barcodes | Santelmo Vasconcelos |
10:30-11:00 | Coffee Break | |
11:00-11:30 | Choosing and using markers for DNA barcodes | Santelmo Vasconcelos |
11:30-13:00 | Developing new DNA barcodes | Santelmo Vasconcelos |
13:00-14:00 | Lunch | CIP Cafeteria |
14:00-15:00 | Analyzing DNA barcodes | Santelmo Vasconcelos |
15:00-16:00 | Exercise: obtaining a dataset, and running the analyses | Santelmo Vasconcelos |
16:00-16:30 | Coffee Break | |
16:30-17:30 | Exercise: obtaining a dataset, and running the analyses | Santelmo Vasconcelos |
17:30 | End of day 2 | |
Day 3 – Thursday 11 April |
||
08:30-09:30 | Phylogeny and species definition by DNA barcodes | Santelmo Vasconcelos |
09:30-10:30 | Exercise: phylogeny and barcode gap | Santelmo Vasconcelos |
10:30-11:00 | Coffee Break | |
11:00-11:30 | Exercise: phylogeny and barcode gap | Santelmo Vasconcelos |
11:30-12:00 | Final Questions | Santelmo Vasconcelos |
12:00-13:00 | Introduction to Metagenomics data, databases and molecular data formats | Marco Cristancho |
13:00-14:00 | Lunch | CIP Cafeteria |
14:00-15:00 | Software for Metagenomics analysis | Marco Cristancho |
15:00-16:00 | Introduction to Galaxy | Marco Cristancho |
16:00-16:30 | Coffee Break | |
16:30-17:30 | Practical: Analysis of Metagenomics Data with Galaxy | Marco Cristancho |
17:30 | End of day 3 | |
18:00-20:00 | Course dinner | |
Day 4 – Friday 12 April |
||
08:30-09:30 | Practical: Analysis of Metagenomics Data with Galaxy | Marco Cristancho |
09:30-10:00 | Q&A on Metagegenomics analysis | Marco Cristancho |
10:00-10:30 | Coffee Break | |
10:30-11:30 | Introduction to genome wide association studies (GWAS) |
Hannele Lindqvist-Kreuze |
11:30-13:00 | Practical: GWAS in polyploid potato using RStudio and GWASPoly | Hannele Lindqvist-Kreuze |
13:00-14:00 | Lunch | CIP Cafeteria |
14:00-14:30 | Q&A on Molecular markers | Hannele Lindqvist-Kreuze |
14:30-15:00 | Introduction to viral metagenomics | Jan Kreuze |
15:00-16:00 | Practical: viral metagenomics using VirusDetect | Jorge Villacorta |
16:00-16:15 | Coffee Break | |
16:15-17:00 | Practical: viral metagenomics using VirusDetect | Jorge Villacorta |
17:00-17:30 | Course wrap-up and feedback | Jan Kreuze / Trainers |
In order to be considered for a place on this course applicants must complete the online application form.
Incomplete applications will NOT be considered.
If you have any queries about the registration process please email Piv Gopalasingam.
The registration fee includes:
- Tea/coffee and lunches will be provided for the duration of the course
- Course dinner on Thursday 11th April
- Use of the course training room and computers
- Course materials provided
- Course certificate