CABANA Workshop: Applied bioinformatics, molecular epidemiology, and the SARS-CoV-2 Pandemic
Date:Tuesday 1 - Friday 4 December 2020
Venue:Virtual - EMBL-EBI, Hinxton, CB10 1SD, United Kingdom
Application opens:Thursday 15 October 2020
Application deadline:Monday 16 November 2020
Participation:Open application with selection
Dates additional information:This workshop will take place between Tuesday 1st - Friday 4th December, each day running for up to 3 hours.
This workshop will provide training on the use of bioinformatic tools for the design and analysis of research and diagnostic molecular assays. Trainees will receive training on viral genome assembly, comparative viral genomics and downstream applications of this data in research and applied contexts. Trainers will focus practical sessions on the analysis of Illumina SARS-CoV-2 sequences.
This online workshop will be delivered on Zoom and comprise a blend of live lectures and hands-on practicals delivered by trainers across Latin America. Certificates will be provided to mark successful completion of this workshop. Sessions on Friday 4th December will be livestreamed on YouTube and made freely available on the platform thereafter. There will be an opportunity to deliver a short presentation about your work to other trainees.
During this course you will learn about:
- Viral genome assembly
- Comparative analysis of viral genomes
- Primer design for qPCR
- Point of care molecular testing
- Standards for reporting of molecular epidemiology studies
This course is suitable for postgraduate (MSc-level and above) scientists within Latin America (excluding Chile and Uruguay) who are working with or generating genomic datasets related to SARS-CoV-2. Ideally, trainees will be working in the field of virology, and performing sequencing and/or analysing viral sequences, especially those within the coronaviridae family. We welcome applications from universities, research centres, hospitals, public health, and private laboratories.
Scientists from underrepresented ethnic and gender groups are especially encouraged to apply for this workshop.
Please note this course will be taught in English, however the trainers are fluent in either Spanish or Portuguese and can offer language support where feasible. Attendance priority will be given to those who have not attended a CABANA event yet.
Undergraduate-level knowledge of biology would be advantageous. You will also require access to a computer for the duration of the workshop. While most practicals are browser-based, a few will require a UNIX environment. Trainees will therefore require a basic knowledge of the Unix command line.
We recommend these free tutorials:
- Basic introduction to the Unix environment: www.ee.surrey.ac.uk/Teaching/Unix
- Introduction and exercises for Linux: https://training.linuxfoundation.org/free-linux-training
Training will be delivered via Zoom; trainees need to ensure they have working audio and microphones.
After this course you should be able to:
- Know the main public repositories for virus sequences
- Analyse genomic sequences of viruses
- Design PCR primers following international recommendations
- Apply international standards for the reporting of molecular epidemiology studies
Day 1 – Tuesday 1 December 2020
|08:45-09:00||Welcome and introductions||Marco Cristancho & Diego Forero|
|09:00-09:45||Sequencing technologies (Theoretical)||Dario Fernandez|
|09:45-10:30||Viral genomes databases – GISAID and other repositories||Priscila Born|
|10:45-11:30||DNA sequence Quality Control (Theoretical - Practical)||Gisele Nunes & Gilderlanio Araujo|
|12:00||End of day 1|
Day 2 – Wednesday 2 December 2020
|09:00-11:00||Assembly of viral genomes (Theoretical - Practical)||Jan Kreuze & Segundo Fuentes|
|12:00||End of day 2|
Day 3 – Thursday 3 December 2020
|09:00-10:00||Comparative analysis of viral genomes (Theoretical - Practical)||Dario Fernandez|
|10:00-11:00||Phylogenomics of viral genomes (Theoretical - Practical)||Dario Fernandez|
|11:45||End of day 3|
Day 4 – Friday 4 December 2020
|08:45-09:30||Primer design for qPCR (Theoretical)||Segundo Fuentes & Ana Perez|
|09:30-10:15||Point of care molecular testing (Point of care isothermal amplification protocols) - LAMP (Theoretical)||Segundo Fuentes - Ana Perez|
|10:30-11:15||Standards for reporting of molecular epidemiology studies - STROBE-ME (Theoretical)||Valentina Gallo|
|11:45-12:00||End of workshop: feedback and certificates||Marco Cristancho & Diego Forero|
This workshop is part supported by the ITV-Fiocruz coronavirus genome initiative.