EMBL-EBI Workshop: Analysis of genome scale data from bulk and single-cell sequencing

Date:

 Monday 19 Friday 23 November 2018

Venue: 

NIBMG - National Institute of Biomedical Genomics, Kalyani, West Bengal, India,  741251, United Kingdom

Application opens: 

Thursday July 12 2018

Application deadline: 

Monday October 15 2018

Participation: 

Open application with selection

Contact: 

Partha P. Majumder

Registration fee: 

£0

Course Overview

This course will provide practical training to researchers in India who are engaged in research or applications that involve analyses of data generated by massively-parallel DNA sequencers using DNA isolated from bulk cells or a single-cell. This course will provide an introduction to the technology, data analysis, and key bioinformatics tools and databases. The content is intended to provide a broad overview of the subject areas, and to highlight key resources, approaches and methodologies. Topics will be delivered using a mixture of lectures, practical sessions and open discussions. Practical work during the course will use small, example data-sets and there will not be scope to analyse personal data.

Audience

This course is open to all scientists and application specialists who are engaged in genome-scale research. Preference will be given to junior-level scientists who can in turn train others in their institutions.

No knowledge of programming is required, but an undergraduate level knowledge of biology is required.

Modules

  • ensemblensembl-genomes
  • expression-atlas-gene-expression-across-species-and-biological-conditions

Learning outcomes

After this course you should be able to:

  • Use basic statistics, UNIX and R commands to navigate the command line, employ and interpret statistics from NGS data.
  • Use a range of bioinformatics software and tools to undertake basic analysis of NGS data
  • State the advantages and limitations of NGS analyses
  • Apply various CNV detection methods
  • Submit, browse and access a range of NGS data available in public repositories using EBI resources

Programme

Day 1 – Monday 19 November
08:30-09:45 Welcome and Introduction Cath Brooksbank
09:45-10:00 Tea break  
10:00-11:00 Statistics: Probability, Distributions, Tests of significance Analabha Basu
11:00-13:00 Overview of NGS Technologies: Platforms, Chemistry, library preparation Arindam Maitra
13:00-14:00 Lunch break
14:00-15:30 Introduction to EMBL-EBI Resources Cath Brooksbank / EBI Team
15:30-17:00 Introduction to ENA and NGS Workflows EBI Webinar & Face-to-face
17:00-17:30 Tea break  
17:30-19:30 Introduction to Ensembl Astrid Gall - Ensembl
19:30 End of Day 1  
Day 2 – Tuesday 20 November
08:30-10:15 Introduction to NGS Data Analysis Nidhan K. Biswas
10:15-10:30 Tea break  
10:30-12:30 Linux: Introduction to the Command Line Nidhan K. Biswas
12:30-13:30 Lunch break  
13:30-15:00 Introduction to NGS file formats, databases and associated tools Nidhan K Biswas / Chitrarpita Das
15:00-15:30 NGS Data QC Swati Jaiswal
15:30-16:30 Exome Sequence data analysis: Alignment, Read Filtering, Variant Calling Nidhan K. Biswas
16:30-17:00 Variant annotation and Variant Filtration Nidhan K. Biswas
17:00-17:15 Tea break  
17:15-18:15 Introduction to R: Commands & Functions Saroj Kant Mohapatra
18:15-19:00 Case Study: Data Processing through R Saroj Kant Mohapatra
19:00 End of Day 2  
Day 3 – Wednesday 21 November
08:30-09:30 Read and Mutation Visualization through IGV Arnab Ghosh
09:30-10:30 Read Visualization in Ensembl Astrid Gall
10:30-10:45 Tea break  
10:45-11:30 Analysis of Genome Structural Alterations: CNV & SV Chitrarpati Das
11:30-12:30 CNV Detection from Genome-WIde Array Data Nidhan K Biswas / Chitrarpati Das
12:30-13:00 CNV Detection from Sequence Data: Paired-end and Mate-pair Nidhan K. Biswas / Chitrapati Das
13:00-14:00 Lunch break  
14:00-16:00 Ensembl workshop - Variation / VEP Astrid Gall
16:00-16:30 Introduction to EVA - Webinar EVA Team / EBI
16:30-17:00 Tea break  
17:00-18:30 Ensembl workshop - Regulation Astrid Gall - Ensembl
19:00 Special dinner  
Day 4 – Thursday 22 November
08:30-09:30 Ensembl workshop - Comparative Genomics Astrid Gall
09:30-10:30 Ensembl workshop - Biomart (Data export tool) Astrid Gall
10:30-10:45 Tea break  
10:45-11:45 Targeted Sequencing and its application Arindam Maitra
11:45-13:00 Bulk RNA Sequencing Analabha Basu
13:00-14:00 Lunch break  
14:00-14:45 Disease Gene Mapping Using Massively Parallel Genome Sequencing / Population Genomics - GenomeAsia Findings Partha P Majumder
14:45-15:15 Cancer focus session: Identification of somatic driver genes in Cancer - Algorithm comparisons Nidhan K. Biswas
15:15-16:45 Circos - Mutation and CNV Visualization Arnab Ghosh
16:45-17:00 Tea break  
17:00-18:00 Introduction to DGVa for CNV data DGVa Team
18:00-19:00 COSMIC data resource for cancer mutations COSMIC Team
19:00 End of day 4  
Day 5 – Friday 23 November
08:30-10:30 Transcriptomic data resources and tools Laura Huerta
10:30-11:00 Tea break  
11:00-12:30 Transcriptomic data resources and tools Laura Huerta
12:30-13:30 Lunch break  
13:30-14:30 Single-cell RNA sequencing and the Human Cell Atlas Laura Huerta / Dani Welter
14:30-16:00 Single cell RNA sequencing: Methods Arindam Maitra
16:00-17:00 Single cell RNA sequencing: Analysis approaches and considerations Nidhan K. Biswas
17:00-17:15 Tea break  
17:15-17:45 Data integration challenges and solutions Partha P Majumder
17:45-18:00 Wrap-up and feedback