Training

This course will provide an introduction to the technology, data analysis, tools and resources for next generation sequencing (NGS) data. The content is intended to provide a broad overview of the subject areas, and to highlight key resources, approaches and methodologies. Topics will be delivered using a mixture of lectures, practical sessions and open discussions. Practical work during the course will use small, example datasets and there will not be scope to analyse personal data.

During this course you will learn about:

• NGS platforms and NGS data files and formats
• NGS bioinformatics workflow steps following sequence generation
• Experimental NGS design and planning for your bioinformatics needs
• NGS for genomics; assembly, alignment, QC and variant calling tools
• NGS for transcriptomics; QC, mapping, visualisation tools
• EMBL-EBI resources: Array Express and Expression Atlas, ENA and the CRAM toolkit

After this course you should be able to:

• Discuss a variety of applications and workflow approaches for NGS technologies
• Use a range of bioinformatics software and tools to undertake basic analysis of NGS data
• Understand the advantages and limitations of NGS analyses
• Submit, browse and access a range of NGS data available in public repositories using EBI resources

The course price includes 3 nights accommodation at the Wellcome Genome Campus Conference Centre and catering for the duration of the course. A bus is provided to take participants to Cambridge Railway Station after the course. Please see Travel to EMBL-EBI for futher information on your journey to the campus.