- Course overview
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- Introduction
- Real-time PCR
- Microarrays
- RNA sequencing
- Biological interpretation of gene expression data
- Genotyping, epigenetic and DNA/RNA-protein interaction methods
- DNA/RNA-protein interactions
- Summary
- Quiz: Check your learning
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- References
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Illumina sequencing
In NGS, vast numbers of short reads are sequenced in a single stroke.
To do this, firstly the input sample must be cleaved into short sections. The length of these sections will depend on the particular sequencing machinery used.
In Illumina sequencing, 100-150bp reads are used. Somewhat longer fragments are ligated to generic adaptors and annealed to a slide using the adaptors. PCR is carried out to amplify each read, creating a spot with many copies of the same read. They are then separated into single strands to be sequenced.
