M-CSA Mechanism and Catalytic Site Atlas

Bile salt-activated lipase (M-CSA:401)

Proteins that are close homologues to the M-CSA reference protein and have the same catalytic residues (highlighted in green, below) probably share the same mechanism. Please also check if the homologue shares the same FunTree Structural Cluster and the same Pfam family, as another measure of similarity.

Legend:
Reference Residue Conserved Residue Non Conserved Residue Outside Alignment Reference Residue not in this sequence

Homologous SwissProt sequences of P30122

Reference Sequence	Homologue	Ala126 (main-N) His453 Ala213 (main-N) Ser212 Asp338 Gly125 (main-N)
P30122	P30122	Ala126 His453 Ala213 Ser212 Asp338 Gly125
P30122	P37967	Ala107 His399 Ala190 Ser189 Glu310 Gly106
P30122	P22394	Ala151 His482 Ala237 Ser236 Glu373 Gly150
P30122	P04058	Gly140 His461 Ala222 Ser221 Glu348 Gly139
P30122	P79066	Ala151 His482 Ala237 Ser236 Glu373 Gly150
P30122	Q6NT32	Ala148 His454 Ala227 Ser226 Glu345 Gly147
P30122	Q6AW46	Gly148 His454 Ala227 Ser226 Glu345 Gly147
P30122	Q6AW47	Ala148 His454 Ala227 Ser226 Glu345 Gly147
P30122	Q5GRG2	Gly148 His454 Ala227 Ser226 Glu345 Gly147
P30122	Q27459	Gly136 His468 Ala217 Ser216 Glu346 Gly135
P30122	P37136	Gly153 His478 Ala235 Ser234 Glu365 Gly152
P30122	Q04791	Gly149 His460 Ala228 Ser227 Glu345 Gly148
P30122	P19835	Ala128 His455 Ala215 Ser214 Asp340 Gly127
P30122	B2D0J5	Ala135 His464 Ala213 Ser212 Glu341 Gly134
P30122	P56161	Gly175 His504 Ala262 Ser261 Glu390 Gly174
P30122	Q64176	Gly144 His467 Ala223 Ser222 Glu354 Gly143
P30122	P38433	Gly136 His468 Ala217 Ser216 Glu346 Gly135
P30122	Q63880	Ser154 His460 Ala233 Ser232 Glu347 Gly153
P30122	Q64573	Gly143 His466 Ala222 Ser221 Glu353 Gly142
P30122	P23795	Gly152 His477 Ala234 Ser233 Glu364 Gly151
P30122	O62763	Gly150 His475 Ala232 Ser231 Glu362 Gly149
P30122	P22303	Gly153 His478 Ala235 Ser234 Glu365 Gly152
P30122	O00748	Ala150 His457 Ala229 Ser228 Glu345 Gly149
P30122	P07140	Gly189 His518 Ala277 Ser276 Glu405 Gly188
P30122	Q9NDG8	Gly139 His477 Ala220 Ser219 Glu347 Gly138
P30122	Q91WG0	Ala152 His459 Ala231 Ser230 Glu347 Gly151
P30122	Q69ZK9	Ser187 His500 Ala266 Gly265 Glu386 Gly186
P30122	Q9DDE3	Gly144 His495 Ala226 Ser225 Glu352 Gly143
P30122	P23953	Gly143 His455 Ser222 Ser221 Glu342 Gly142
P30122	P25727	Ala132 His470 Ala211 Ser210 Asp341 Gly131
P30122	Q99K10	Ser203 His525 Ala282 Gly281 Glu411 Gly202
P30122	P81908	Gly117 His438 Ala199 Ser198 Glu325 Gly116
P30122	Q5XG92	Ala143 His467 Ala222 Ser221 Glu353 Gly142
P30122	Q04457	Gly117 His452 Ala199 Ser198 Glu319 Gly116
P30122	Q8QZR3	Gly149 His456 Ala228 Ser227 Glu344 Gly148
P30122	P14943	Gly123 His430 Ala202 Ser201 Glu318 Gly122
P30122	P12337	Gly143 His467 Ala222 Ser221 Glu353 Gly142
P30122	P36196	Gly146 His633 Ala228 Ser227 Glu520 Gly145
P30122	Q8I034	Ala148 His454 Ala227 Ser226 Glu346 Gly147
P30122	O62760	Gly145 His466 Ala227 Ser226 Glu353 Gly144
P30122	P01267	Ala2307 His2620 Gly2388 Arg2387 Asp2515 Ala2306
P30122	Q62888	Ser187 His500 Ala266 Gly265 Glu386 Gly186
P30122	P23141	Gly143 His468 Ala222 Ser221 Glu354 Gly142
P30122	Q8NFZ3	Ser176 His489 Ala255 Gly254 Glu375 Gly175
P30122	Q6UWW8	Ala151 His460 Ala230 Ser229 Glu347 Gly150
P30122	Q91WU0	Gly143 His466 Ala222 Ser221 Glu353 Gly142
P30122	P21927	Gly124 His445 Ala206 Ser205 Glu332 Gly123
P30122	Q8NFZ4	Ser187 His500 Ala266 Gly265 Glu386 Gly186
P30122	Q8N2Q7	Ser200 His522 Ala279 Gly278 Glu408 Gly199
P30122	Q8N0W4	Ser176 His489 Ala255 Gly254 Glu375 Gly175
P30122	Q29550	Gly144 His467 Ala223 Ser222 Glu354 Gly143
P30122	Q5RCL7	Ala149 His458 Ala228 Ser227 Glu345 Gly148
P30122	P16303	Gly143 His466 Ala222 Ser221 Glu353 Gly142
P30122	Q04456	Gly118 His451 Ala200 Ser199 Glu320 Gly117
P30122	P32749	Ser145 His466 Ala227 Ser226 Glu353 Gly144
P30122	P21836	Gly153 His478 Ala235 Ser234 Glu365 Gly152
P30122	Q8BYM5	Ser187 His500 Ile266 Gly265 Glu386 Gly186
P30122	Q8R0W5	Ala150 His475 Ala229 Ser228 Glu360 Gly149
P30122	P10959	Gly143 His453 Ala222 Ser221 Glu340 Gly142
P30122	Q869C3	Gly280 His600 Ala361 Ser360 Glu486 Gly279
P30122	Q8VCU1	Ser154 His460 Ala233 Ser232 Glu347 Gly153
P30122	Q62765	Ser203 His525 Ala282 Gly281 Glu411 Gly202
P30122	B0F2B4	Ser185 His562 Ala264 Gly263 Glu396 Gly184
P30122	Q9NZ94	Ser210 His523 Ile289 Gly288 Glu409 Gly209
P30122	P0C6R3	Ala143 His465 Ser222 Ser221 Glu353 Gly142
P30122	Q8BK48	Ala150 His457 Ala229 Ser228 Glu345 Gly149
P30122	Q8VCT4	Gly143 His466 Ala222 Ser221 Glu353 Gly142
P30122	P06276	Gly145 His466 Ala227 Ser226 Glu353 Gly144
P30122	P18167	Ala128 His466 Ala207 Ser206 Asp337 Gly127
P30122	Q63108	Gly143 His466 Ala222 Ser221 Glu353 Gly142
P30122	Q8VCC2	Gly143 His466 Ala222 Ser221 Glu353 Gly142
P30122	Q29499	Gly123 His448 Ala205 Ser204 Glu335 Gly122
P30122	P07882	Ala128 His455 Ala215 Ser214 Asp340 Gly127
P30122	Q07085	Gly127 His446 Ala209 Ser208 Glu331 Gly126
P30122	Q62889	Ser210 His523 Ile289 Gly288 Glu409 Gly209
P30122	P25726	Ala129 His467 Ala208 Ser207 Asp338 Gly128
P30122	Q63010	Gly143 His466 Ala222 Ser221 Glu353 Gly142
P30122	Q3T930	Ala30 His336 Ala109 Ser108 Glu227 Gly29
P30122	Q03311	Gly146 His467 Ala228 Ser227 Glu354 Gly145
P30122	Q64285	Ala128 His455 Ala215 Ser214 Asp340 Gly127
P30122	Q9XTG1	not aligned Ser490 Ser234 Gly233 Pro370 not aligned
P30122	Q47M62	Ala108 His415 Ala186 Ser185 Glu319 Gly107
P30122	Q5UR02	Ala131 His451 Ala218 Ser217 Glu337 Gly130
P30122	O08710	Met2305 His2618 Ser2386 Arg2385 Asp2513 Thr2304
P30122	P18142	Ala136 His440 Ala214 Ser213 Glu338 Gly135
P30122	P25725	Ala129 His467 Ala208 Ser207 Asp338 Gly128
P30122	P32750	Gly50 not aligned Ala132 Ser131 not aligned Gly49
P30122	P32751	Gly50 not aligned Ala132 Ser131 not aligned Gly49
P30122	P06882	Val2307 His2620 Gly2388 Arg2387 Asp2515 Thr2306
P30122	P21837	Ala138 His443 Ala216 Ser215 Glu340 Gly137
P30122	Q9UKY3	Gly144 not aligned Val223 Ser222 not aligned Gly143
P30122	P01266	Met2306 His2619 Gly2387 Arg2386 Asp2514 Thr2305
P30122	P32753	Ser50 not aligned Ala132 Ser131 not aligned Gly49
P30122	D4B1N9	Gly150 His480 Ala236 Ser235 Glu367 Gly149
P30122	D4ASH1	Gly128 His448 Ser216 Ser215 Asp345 Gly127
P30122	P32752	Gly50 not aligned Ala132 Ser131 not aligned Gly49
P30122	A1CFK9	Ala171 His472 Ala259 Ser258 Glu380 Gly170
P30122	A0A075TXZ3	Ala176 His477 Ala264 Ser263 Glu385 Gly175
P30122	O94493	not aligned His456 Ala217 Ser216 Glu346 Gly127
P30122	D4AZ78	Gly108 His401 Ala197 Ser196 Glu314 Gly107
P30122	Q96VC9	Ser118 His409 Ala201 Ser200 Glu313 Gly117
P30122	Q99156	Gly106 His392 Ala194 Ser193 Asp303 Gly105
P30122	Q64419	Ala149 His459 Ala228 Ser227 Glu345 Gly148
P30122	P08171	Ala131 His466 Ala210 Ser209 Asp340 Gly130
P30122	P20261	Gly139 His464 Ala225 Ser224 Glu356 Gly138
P30122	P32946	Gly138 His463 Ala224 Ser223 Glu355 Gly137
P30122	P32947	Gly139 His464 Ala225 Ser224 Glu356 Gly138
P30122	Q92035	Gly150 His471 Ala232 Ser231 Glu358 Gly149
P30122	P35502	Gly136 His463 Ala215 Ser214 Glu339 Gly135
P30122	D2D3B6	Gly155 His448 Ala241 Ser240 Glu356 Gly154
P30122	P12992	Gly141 His465 Ala221 Ser220 Glu351 Gly140
P30122	P47982	Ala129 His464 Ala208 Ser207 Asp338 Gly128
P30122	O42275	Gly144 His494 Ala226 Ser225 Glu352 Gly143
P30122	P17573	Ala151 His482 Ala237 Ser236 Glu373 Gly150
P30122	O16168	Ala132 His470 Ala211 Ser210 Asp341 Gly131
P30122	Q86GC8	Gly247 His567 Ala328 Ser327 Glu453 Gly246
P30122	P86325	Ala108 His415 Ala186 Ser185 Glu319 Gly107
P30122	P32948	Gly139 His464 Ala225 Ser224 Glu356 Gly138
P30122	O62761	Gly145 His466 Ala227 Ser226 Glu353 Gly144
P30122	O46421	Gly143 His467 Ala222 Ser221 Glu354 Gly142
P30122	Q08662	Ala129 His464 Ala208 Ser207 Asp338 Gly128
P30122	Q92081	Gly19 not aligned Ala100 Ser99 Glu226 Gly18
P30122	Q05487	Gly126 Met462 Thr205 Ser204 Asn335 Gly125
P30122	P07692	Gly143 His464 Ala225 Ser224 Glu351 Gly142
P30122	Q95000	Gly32 His357 Ala113 Ser112 Glu244 Gly31
P30122	P81429	Ala134 not aligned not aligned not aligned not aligned Gly133
P30122	P33438	Met169 Leu504 Gly247 Ala246 Ala383 Glu168
P30122	Q95001	Gly19 not aligned Ala100 Ser99 Glu224 Gly18
P30122	Q01470	Gly111 His402 Gly189 Ser188 Glu307 Gly110
P30122	P35501	Gly136 His463 Ala215 Ser214 Glu339 Gly135
P30122	O16173	Ala132 His470 Ala211 Ser210 Asp341 Gly131
P30122	W4VSJ0	Gly143 His471 Val224 Ser223 Glu354 Gly142
P30122	P16854	Gly110 His442 Ala192 Ser191 Glu324 Gly109
P30122	O16169	Ala129 His467 Ala208 Ser207 Asp338 Gly128
P30122	P23654	Ser463 not aligned Ala549 Arg548 Glu679 Glu462
P30122	P32949	Gly139 His464 Ala225 Ser224 Glu356 Gly138
P30122	O16171	Ala129 His467 Ala208 Ser207 Asp338 Gly128
P30122	O16170	Ala129 His467 Ala208 Ser207 Asp338 Val128
P30122	O16172	Ala129 His467 Ala208 Ser207 Asp338 Gly128
P30122	Q27677	Gly167 His496 Ala254 Ser253 Glu382 Gly166