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Figure 8.
Figure 8. Schematic of dUTPase-substrate interactions
showing the structural basis for the exquisite specificity for
dUTP that requires all three subunits. Hydrogen bonds (<3.4 å )
are shown (dashed lines) with the donor-acceptor atom distance
beneath the protein atom label. Hydrophobic interactions (wavy
lines) are shown only for the key residues Tyr82, which packs
against the deoxyribose, and Phe135, which stacks above the
bound uracil base. Uracil and deoxyribose are primarily
recognized by one subunit (gold rectangles), phosphate groups by
the adjacent subunit (purple rectangles), whereas the bound
substrate is capped by residues from the C-terminal tail of a
third subunit (blue rectangles).
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