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Figure 8.
Variability in the P[i] binding pocket.a, transparent surface
representation of the human sperm model in the vicinity of the
active site, with the loop containing residues 189–192 shown
in sticks representation for the human sperm model with carbons
in cyan, along with the equivalent loop from human placental
GAPDH (1U8F) in light magenta, human liver GAPDH (1ZNQ) in
purple, and rabbit muscle GAPDH (1J0X) in white. The Glc-3-P
structure is shown with green carbons demonstrating the P[s]
site, and a model of 3-chlorolactaldehyde (CL) is shown in the
P[i] site, with carbons in blue. b, intersubunit selectivity
cleft of the His-GAPDS tetramer is shown in schematic diagram
(D, blue; A, yellow; B, red), with the residues that differ in
sequence shown as sticks. The equivalent loops in human GAPDH
are shown in cyan.
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