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Figure 8.
Figure 8 Schematic diagram of the active-site structures of the
2-hydroxyoctanoate and 3-indolelactate complexes of the G81A
mutant form of MDH-GOX2. Hydrogen-bonding interactions between
the carboxylate and hydroxyl O atoms and nearby side chains or
water molecules are shown as dashed lines and the distances are
in Å. Residues making hydrophobic contact to the ligand
are indicated as shown at the bottom right. C atoms are black, O
atoms red and N atoms cyan. Covalent bonds within the ligand are
drawn with pink lines, while those within the protein are drawn
with orange lines. (a) The 2-hydroxyoctanoate-G81A complex. The
ligand is labeled `Octanoate'. (b) The
(D,L)-2-hydroxy-3-indolelactate-G81A complex. The ligand is
labeled `ILAC'. This diagram was prepared using the program
LIGPLOT (Wallace et al., 1995[Wallace, A. C., Laskowski, R. A. &
Thornton, J. M. (1995). Protein Eng. 8, 127-134.]).
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