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Figure 8.
Figure 8. Proposed catalytic mechanism of
6-aminohexanoate-dimer hydrolase. In this model, enzyme
catalysis proceeds according to the following steps: (i)
Ald-induced transition from open to closed form, (ii)
nucleophilic attack of Ser112 to Ald and formation of
tetrahedral intermediate, (iii) formation of acyl enzyme and
transition to open form, (iv) deacylation (formation of
tetrahedral intermediate and regeneration of free enzyme). (a)
Free enzyme, (d) acyl enzyme, and (e) tetrahedral intermediate
are present as open forms, and (b) enzyme + substrate and (c)
tetrahedral intermediate are present as closed forms. Figure
8. Proposed catalytic mechanism of 6-aminohexanoate-dimer
hydrolase. In this model, enzyme catalysis proceeds according to
the following steps: (i) Ald-induced transition from open to
closed form, (ii) nucleophilic attack of Ser112 to Ald and
formation of tetrahedral intermediate, (iii) formation of
acyl enzyme and transition to open form, (iv) deacylation
(formation of tetrahedral intermediate and regeneration of free
enzyme). (a) Free enzyme, (d) acyl enzyme, and (e) tetrahedral
intermediate are present as open forms, and (b) enzyme +
substrate and (c) tetrahedral intermediate are present as closed
forms.
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