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Figure 7.
Comparison of arrestin position in clathrin superposition.A,
top view of the clathrin terminal domain shows two distinct
interactions with arrestin2L, which are depicted as red ribbons
(left). The side view of arrestin2L shows two separated clathrin
binding sites, the LϕXϕ(D/E) motif and splice loop (right, red
dotted lines). B, side view; arrestins (cyan, low resolution;
wheat, high resolution) near the clathrin binding box (red
ribbon) do not superimpose upon the superposition of clathrin
(green) from the different crystal forms. Also shown is the
second arrestin with a splice loop. Each end point of flanking
regions in unstructured C-terminal loop is marked and connected
as blue balls and a dotted line for the N-terminal area and red
balls and a dotted line for the C-terminal area. C, top view;
same as B but rotated 90° in a plane and looking down at
approximate rotation axis relating the two arrestins. D,
electron microscopy image of a clathrin barrel with a set of
clathrin terminal domains shown in red (from Edeling et al.
(59)). The enlarged inset is a schematic representation of the
six terminal domains within the cluster, depicting a distance of
∼64 Å between terminal domains. The distance between
terminal domains was measured using the measurement function in
PyMol using PDB code 1XI4 for the clathrin D6 coat (48).
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