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Figure 7.
Figure 7. Interaction between LSD1-SWIRM and the Peptides of
the Histone Sequences
(A–F) Biotinylated peptides were
immobilized onto the streptavidin chips, and 64 μM (blue), 32
μM (green), 16 μM (cyan), or 8 μM (magenta) LSD1-SWIRM
protein solution was passed over the chip at a 20 μl/min flow
rate for 2 min. Biotinylated peptides used are (A) H3(1–20),
(B) H3(1–20)-K4diMe, (C) H3(1–20)-K9diMe, (D)
H3(1–20)-R8E/T11K, (E) H3(122–135), and (F) H4(1–15). The
x axis indicates the time course. Each injection starts at 0 s
and ends at 120 s. The y axis indicates the difference in the
resonance units between the peptide bound and peptide unbound
flow cells.
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